Intestinal Absorption and Lipoprotein Transport of (ω-3) Eicosapentaenoic Acid
Intestinal Absorption and Lipoprotein Transport of (ω-3) Eicosapentaenoic Acid
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(ω-3)二十碳五烯酸的肠道吸收和脂蛋白转运
DOI：
10.1093/jn/115.2.219
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发表时间：
1985
影响因子：
4.2
通讯作者：
G. Vahouny
中科院分区：
文献类型：
--
作者：
I. Chen;S. Subramaniam;M. Cassidy;A. J. Sheppard;G. Vahouny
通过手术在成年雄性大鼠左胸淋巴管中提供引流导管，并留置胃导管以持续输注盐水-葡萄糖。禁食过夜后，动物单次灌胃含以下1- 14 C标记脂肪酸之一的水乳剂：油酸、花生四烯酸或二十碳五烯酸和[1，2 - 3 H]胆固醇。通过在24小时采集期内淋巴中出现放射性来估计吸收，并在24小时样品中测定淋巴脂蛋白分布和脂蛋白脂质分布。虽然二十碳五烯酸和花生四烯酸的吸收率有明显的差异，这些酸在淋巴液中的整体外观（24小时后）是定量相当于油酸。此外，从每种脂肪酸培养基中吸收的胆固醇在数量上相似。各脂肪酸在主要淋巴脂蛋白中的分布相似，乳糜微粒和极低密度脂蛋白（VLDL）组分中回收率为93-95%。此外，在这些脂蛋白中，三种脂肪酸中的每一种的85-91%以甘油三酯的形式回收。与这两种多不饱和脂肪酸，有更大的掺入磷脂和它们的前体偏甘油酯比油酸酯。这些研究表明，未酯化的二十碳五烯酸被有效地吸收到淋巴系统中，并且具有与更典型的单不饱和脂肪酸和多不饱和脂肪酸相当的淋巴脂蛋白和脂质分布。
Adult male rats were surgically provided with a drainage catheter in the left thoracic lymphatic channel and an indwelling gastric catheter for constant infusion of saline-glucose. After an overnight fast, animals were given a single gastric dose of an aqueous emulsion containing one of the following 1-14C-labeled fatty acids: oleic, arachidonic or eicosapentaenoic acid, and [1,2-3H]cholesterol. Absorption was estimated by the appearance of radioactivity in lymph over a 24-h collection period, and the lymph lipoprotein distributions and lipoprotein lipid distribution were determined in the 24-h samples. Although there were apparent differences in the rates of eicosapentaenoic and arachidonic acid absorption, the overall appearance (after 24 h) of these acids in lymph was quantitatively equivalent to that of oleate. Furthermore, cholesterol absorption from each fatty acid medium was quantitatively similar. The distributions of each fatty acid among major lymph lipoproteins were similar with 93-95% recovered in chylomicrons and very low density lipoprotein (VLDL) fractions. Furthermore in these lipoproteins, 85-91% of each of the three fatty acids was recovered as triglycerides. With both polyunsaturated fatty acids, there was greater incorporation into phospholipids and their precursor partial glycerides than with oleate. These studies suggest that unesterified eicosapentaenoic acid is absorbed efficiently into the lymphatic system, and has lymph lipoprotein and lipid distribution comparable to those of the more typical mono- and polyunsaturated fatty acids.