THE INDUCTION OF BENZO[A]PYRENE-3-MONO-OXYGENASE BY SINGLET OXYGEN IN LIVER-CELL CULTURE IS MEDIATED BY OXIDATION-PRODUCTS OF HISTIDINE
THE INDUCTION OF BENZO[A]PYRENE-3-MONO-OXYGENASE BY SINGLET OXYGEN IN LIVER-CELL CULTURE IS MEDIATED BY OXIDATION-PRODUCTS OF HISTIDINE
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DOI：
10.1016/0009-2797(80)90057-5
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发表时间：
1980-01-01
影响因子：
5.1
通讯作者：
FRANCIS, JE
中科院分区：
文献类型：
--
作者：
PAINE, AJ;FRANCIS, JE
通过含有15 μ M核黄素的培养基的温和照射光化学产生O2的激发态，导致源自肝脏的细胞系[大鼠肝ARL细胞和小鼠肝癌Hepa-1细胞]中苯并[a]芘-3-单加氧酶的典型诱导。单加氧酶的诱导不是由于O2的激发态直接激活细胞内的诱导机制，而是由于形成稳定诱导物的培养基组分的氧化。氧化的成分是His。含有核黄素的培养基的温和光照将通常不是单加氧酶诱导剂的培养基的生理组分转化为与经典诱导剂一样有效的诱导剂的化合物。发现单线态O2将氧化成一个强大的诱导细胞成分是兼容的假设，激发态的O2和它们的氧化产物可能在细胞色素P-450和相关的酶活性的诱导许多化学无关的诱导剂中发挥核心作用。
The photochemical generation of excited states of O2 by the mild illumination of culture medium containing 15 .mu.M riboflavine results in a typical induction of benzo[a]pyrene-3-mono-oxygenase in cell lines [rat liver ARL cells and mouse hepatoma Hepa-1 cells] derived from liver. The induction of the mono-oxygenase is not due to an excited state of O2 directly activating the inducing mechanism inside the cell, but is due to the oxidation of a component of the culture medium forming a stable inducer. The component oxidized is His. The mild illumination of culture medium containing riboflavine converts a physiological component of the medium which is not normally an inducer of the mono-oxygenase into a compound which is as effective an inducer as the classical inducer. The finding that singlet O2 will oxidize a cell constituent into a powerful inducer is compatible with the hypothesis that excited states of O2 and their oxidation products may play a central role in the induction of cytochrome P-450 and associated enzyme activities by many chemically unrelated inducers.