MANGANESE (MN) TRANSPORT ACROSS THE RAT-BLOOD BRAIN BARRIER - SATURABLE AND TRANSFERRIN-DEPENDENT TRANSPORT MECHANISMS
MANGANESE (MN) TRANSPORT ACROSS THE RAT-BLOOD BRAIN BARRIER - SATURABLE AND TRANSFERRIN-DEPENDENT TRANSPORT MECHANISMS
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DOI：
10.1016/0361-9230(94)90204-6
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发表时间：
1994-01-01
影响因子：
3.8
通讯作者：
GANNON, M
中科院分区：
文献类型：
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作者：
ASCHNER, M;GANNON, M
采用单毛细血管通过技术，研究了锰（Mn）跨血脑屏障（BBB）的转运。大鼠脑中Mn 2 + [0-1000 μ M]积累的初始速率测量（15 s）通过1/v与1/s图和v与[s]图清楚地表明了饱和动力学。与单独注射Mn 2+相比，以1：10摩尔比新鲜混合的Mn 2+与转铁蛋白的颈总动脉注射没有导致Mn脑水平的初始速率的显著变化。然而，当Mn 2+孵育在25度-C的转铁蛋白的存在下，在1：10的比例，最多5天，颈总动脉注射前，由大脑的Mn摄取的初始速率是孵育时间依赖性的，线性增加与延长孵育。这些结果表明，二价锰运输到大脑的饱和成分代表，但跨血脑屏障的锰运输机制之一。Mn的第二个转运系统可能通过转铁蛋白缀合的Mn转运系统发生。
Using a single capillary pass technique, the transport of manganese (Mn) across the rat blood-brain barrier (BBB) was characterized. Initial rate measurements (15 s) of Mn2+ [0-1000 muM] accumulation in rat brains clearly indicated saturation kinetics by both 1/v vs. 1/s plots, and plots of v vs. [s]. Common carotid injection of freshly mixed Mn2+ with transferrin at a 1:10 molar ratio did not result in a significant change in the initial rate of Mn brain levels compared with injection of Mn2+ alone. However, when Mn2+ was incubated at 25-degrees-C in the presence of transferrin at a 1:10 ratio for up to 5 days prior to common carotid injection, the initial rate of Mn uptake by brain was incubation-time-dependent, increasing linearly with prolonged incubations. These findings suggest that the saturable component of divalent Mn transport into brain represents but one of the transport mechanisms for Mn across the BBB. A second transport system for Mn may occur by a transferrin-conjugated Mn transport system.