A Preliminary Study Comparing the Use of Allogenic Chondrogenic Pre-Differentiated and Undifferentiated Mesenchymal Stem Cells for the Repair of Full Thickness Articular Cartilage Defects in Rabbits
A Preliminary Study Comparing the Use of Allogenic Chondrogenic Pre-Differentiated and Undifferentiated Mesenchymal Stem Cells for the Repair of Full Thickness Articular Cartilage Defects in Rabbits
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DOI：
10.1002/jor.21413
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发表时间：
2011-09-01
影响因子：
2.8
通讯作者：
Kamarul, Tunku
中科院分区：
文献类型：
--
作者：
Dashtdar, Havva;Rothan, Hussin A.;Kamarul, Tunku
软骨分化间充质干细胞（CMSC）已被证明在体外产生上级软骨表达标志物。然而，这些细胞在体内的使用尚未得到充分探索。在这项研究中，在体内评估软骨修复潜力之间的同种异体衍生的软骨预分化间充质干细胞和未分化的骨髓间充质干细胞（MSCs）进行了比较。在12只家兔（n = 12）的股骨内侧髁上造成双侧全层软骨缺损。将家兔分为两组。在第一组中，使用藻酸盐包封的MSC修复右膝的缺损，而在第二组中，使用CMSCs。在植入后3个月和6个月处死动物，并对修复膝关节和对照膝关节进行评估。通过测量糖胺聚糖（GAG）/总蛋白含量进行定量分析。6个月时，移植膝关节的平均Brittberg评分高于未治疗膝关节（p < 0.05）。GAG的定量分析与这些结果一致。组织学和免疫组织化学分析表明，透明样软骨再生的移植网站。在3个月和6个月时，在对侧膝关节之间观察到基于O 'Drivel组织学分级的组织学评分之间的显著差异（p < 0.05）。当比较用MSC和CMSC处理的缺损部位时，在Britberg、O 'Drivel评分和GAG/总蛋白含量之间没有观察到显著差异（p > 0.05）。该研究表明，与未治疗的软骨缺损相比，MSC或CMSC的使用产生了更好的上级愈合。然而，两种细胞产生了相当的治疗结果。(C)2011骨科研究学会。由威利期刊公司出版J Orthop Res 29：1336-1342，2011
Chondrogenic differentiated mesenchymal stem cells (CMSCs) have been shown to produce superior chondrogenic expression markers in vitro. However, the use of these cells in vivo has not been fully explored. In this study, in vivo assessment of cartilage repair potential between allogenic-derived chondrogenic pre-differentiated mesenchymal stem cells and undifferentiated MSCs (MSCs) were compared. Bilateral full thickness cartilage defects were created on the medial femoral condyles of 12 rabbits (n = 12). Rabbits were divided into two groups. In one group, the defects in the right knees were repaired using alginate encapsulated MSCs while in the second group, CMSCs were used. The animals were sacrificed and the repaired and control knees were assessed at 3 and 6 months after implantation. Quantitative analysis was performed by measuring the Glycosaminoglycans (GAGs)/total protein content. The mean Brittberg score was higher in the transplanted knees as compared to the untreated knee at 6 months (p < 0.05). Quantitative analysis of GAGs was consistent with these results. Histological and immunohistochemical analysis demonstrated hyaline-like cartilage regeneration in the transplanted sites. Significant differences between the histological scores based on O'Driscoll histological grading were observed between contralateral knees at both 3 and 6 months (p < 0.05). No significant differences were observed between the Britberg, O'Driscoll scores, and GAGs/total protein content when comparing defect sites treated with MSC and CMSC (p > 0.05). This study demonstrates that the use of either MSC or CMSC produced superior healing when compared to cartilage defects that were untreated. However, both cells produced comparable treatment outcomes. (C) 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29: 1336-1342, 2011