ParABS system in chromosome partitioning in the bacterium Myxococcus xanthus.
ParABS system in chromosome partitioning in the bacterium Myxococcus xanthus.
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DOI：
10.1371/journal.pone.0086897
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Iniesta AA
中科院分区：
文献类型：
--
作者：
Iniesta AA
染色体分离是真核细胞和原核细胞的一项基本细胞功能。ParABS系统是许多细菌物种中染色体分配中的有丝分裂样过程的基本参与者。这项工作表明，社会细菌黄色粘球菌也使用ParABS系统进行染色体分离。该菌原核基因组全长9.1Mb，在复制起点附近含有22个parS序列。在体外，苍耳ParB优先结合共有的parS序列。ParB和帕拉对M. xanthus，如新月柄杆菌，但与许多其他细菌不同。缺乏ParB导致无核细胞、染色体分离缺陷和活力丧失。对帕拉亚细胞定位的分析表明，它在所有细胞中聚集在两极，在一些细胞中，聚集在两个染色体DNA团之间的无DNA细胞分裂平面上。这种帕拉定位模式依赖于ParB而不依赖于FtsZ。ParB抑制帕拉与DNA的非特异性相互作用，并且只有当ParB耗尽时，帕拉才与染色体DNA共定位。ParB的亚细胞定位表明，单个ParB-parS复合物定位在类核的边缘，靠近极性帕拉簇，第二个ParB-parS复合物在parS复制发生后迁移到相对的类核边缘，靠近另一个极性帕拉簇。
Chromosome segregation is an essential cellular function in eukaryotic and prokaryotic cells. The ParABS system is a fundamental player for a mitosis-like process in chromosome partitioning in many bacterial species. This work shows that the social bacterium Myxococcus xanthus also uses the ParABS system for chromosome segregation. Its large prokaryotic genome of 9.1 Mb contains 22 parS sequences near the origin of replication, and it is shown here that M. xanthus ParB binds preferentially to a consensus parS sequence in vitro. ParB and ParA are essential for cell viability in M. xanthus as in Caulobacter crescentus, but unlike in many other bacteria. Absence of ParB results in anucleate cells, chromosome segregation defects and loss of viability. Analysis of ParA subcellular localization shows that it clusters at the poles in all cells, and in some, in the DNA-free cell division plane between two chromosomal DNA masses. This ParA localization pattern depends on ParB but not on FtsZ. ParB inhibits the nonspecific interaction of ParA with DNA, and ParA colocalizes with chromosomal DNA only when ParB is depleted. The subcellular localization of ParB suggests a single ParB-parS complex localized at the edge of the nucleoid, next to a polar ParA cluster, with a second ParB-parS complex migrating after the replication of parS takes place to the opposite nucleoid edge, next to the other polar ParA cluster.
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DOI：
10.1073/pnas.182539899
发表时间：
2002-10-29
影响因子：
11.1
作者：
Dworkin, J;Losick, R
通讯作者：
Losick, R
影响因子：
3.2
作者：
Bartosik, AA;Lasocki, K;Jagura-Burdzy, G
通讯作者：
Jagura-Burdzy, G
影响因子：
3.2
作者：
Charaka, Vijay Kumar;Misra, Hari S.
通讯作者：
Misra, Hari S.
影响因子：
4.4
作者：
Gomez-Santos, Nuria;Treuner-Lange, Anke;Munoz-Dorado, Jose
通讯作者：
Munoz-Dorado, Jose
影响因子：
16
作者：
Easter, J;Gober, JW
通讯作者：
Gober, JW