A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis.
A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis.
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DOI：
10.1039/c5lc01117e
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发表时间：
2016-02-07
期刊：
影响因子：
6.1
通讯作者：
He M
中科院分区：
文献类型：
--
作者：
Zhao Z;Yang Y;Zeng Y;He M
富含一组肿瘤抗原的肿瘤来源的循环外泌体已被认为是通过微创程序进行癌症诊断的有前途的生物标志物来源。定量精确定位外泌体肿瘤标志物是有吸引力的，但具有挑战性。在这项研究中，我们开发了一种简单的微流控方法（ExoSearch），它提供了丰富的血浆外泌体的制备，用于使用免疫磁珠进行原位多重检测。ExosSearch芯片提供稳健的连续流动设计，可在广泛的制备体积（10 μL至10 mL）范围内定量分离和释放血浆外泌体。我们使用ExoSearch芯片通过使用卵巢癌患者血浆的训练集多重测量三种外泌体肿瘤标志物（CA-125、EpCAM、CD 24）来进行基于血液的卵巢癌诊断，其显示出显著的诊断能力（a.u.c. = 1.0，p = 0.001），并与标准Bradford试验相当。这项工作为外泌体在临床癌症诊断中的应用以及基础外泌体研究提供了一个必不可少的平台。
Tumor-derived circulating exosomes, enriched with a group of tumor antigens, have been recognized as a promising biomarker source for cancer diagnosis via less invasive procedure. Quantitatively pinpointing exosome tumor markers is appealing, yet challenging. In this study, we developed a simple microfluidic approach (ExoSearch) which provides enriched preparation of blood plasma exosomes for in-situ, multiplexed detection using immunomagnetic beads. The ExosSearch chip offers robust, continuous-flow design for quantitative isolation and release of blood plasma exosomes in a wide range of preparation volumes (10 μL to 10 mL). We employed the ExoSearch chip for blood-based diagnosis of ovarian cancer by multiplexed measurement of three exosomal tumor markers (CA-125, EpCAM, CD24) using a training set of ovarian cancer patient plasma, which showed significant diagnostic power (a.u.c. = 1.0, p = 0.001) and was comparable with standard Bradford assay. This work provides an essentially needed platform for utilization of exosomes in clinical cancer diagnosis, as well as fundamental exosome research.