Research on critical technology of micro/nano bioparticles manipulation platform based on light-induced dielectrophoresis
Research on critical technology of micro/nano bioparticles manipulation platform based on light-induced dielectrophoresis
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DOI：
10.1007/s11431-009-0104-6
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发表时间：
2009-09
期刊：
影响因子：
--
通讯作者：
Z. Ni;Hong Yi;Shucun Zhu;Chunfeng Song
中科院分区：
文献类型：
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作者：
Z. Ni;Hong Yi;Shucun Zhu;Chunfeng Song
在研究介电微纳生物微粒的研究现状和存在问题的基础上，对光诱导介电微纳生物微粒操纵的理论基础和模型模拟进行了探讨。得到了不同高度的电场和电泳力的空间分布。在此基础上，完成了微操作系统的核心部件，即芯片的光导层的选材、制作工艺和性能分析测试。得到了悬浮体的电压降和有效电压频谱。最后，将机器视觉检测与实时跟踪系统相结合，建立了基于光诱导介电泳法的微纳米生物颗粒操纵平台，实现了对微纳米生物颗粒的快速采集、输送、分离等操作。这为基于微流控芯片的重大疾病的快速、准确、低成本检测和早期诊断提供了依据。
On the basis of the research on the status and problems of micro/nano bio-particles manipulation using dielectrophoresis, the theoretical basis and the model simulation of micro/nano bio-particles manipulation using light-induced dielectrophoresis were discussed. The space distribution of electric field and dielectrophoresis forces in different heights were also obtained. On this basis, the core component of the micro manipulation system, that is, photoconductive layer of the chip, was completed in the material selection, fabricating process and performance analysis testing. Then the voltage drop of the suspension and the effective voltage frequency spectrum were obtained. Finally, by combining the machine vision detection with real-time tracking system, the micro/nano bio-particles manipulation platform based on the light-induced dielectrophoreisis was established, and then the manipulations for micro/nano bio-particles, such as quick collection, transport, separation, were implemented. This provided a basis for rapid, accurate, and low-cost detection of serious diseases based on the micro-fluidic biochip and early diagnosis.