Abscisic acid (ABA) inhibition of lateral root formation involves endogenous ABA biosynthesis in Arachis hypogaea L.
Abscisic acid (ABA) inhibition of lateral root formation involves endogenous ABA biosynthesis in Arachis hypogaea L.
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DOI：
10.1007/s10725-009-9365-0
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发表时间：
2009-06-01
影响因子：
4.2
通讯作者：
Li, Ling
中科院分区：
文献类型：
--
作者：
Guo, Dongliang;Liang, Jianhua;Li, Ling
ABA在花生幼苗胚后发育中起重要作用。本研究结果表明，外源ABA浓度为10 μ mol l（-1）时，侧根（LRs）数量减少，幼苗发育迟缓。这种效应被25 μ mol l（-1）萘普生（ABA生物合成抑制剂）所消除。缺乏ABA生物合成的拟南芥突变体nced3表现出更多和更长的LRs表型。结果表明，ABA对花生根分枝的抑制呈剂量依赖性。ABA处理的幼苗内源ABA含量高于对照和萘普生处理的幼苗。RT-PCR结果表明，外源ABA显著上调了花生中ABA生物合成途径关键基因AhNCED1的表达。ABA处理2天后，AhNCED1 mRNA水平开始升高。本研究结果表明，ABA通过增加内源ABA含量抑制花生LR发育。
ABA has been found to play a significant role in post-embryonic developmental in peanut seedlings. The results from the current study indicate that in the presence of exogenous 10 mu mol l(-1) ABA, lateral roots (LRs) number decreased and seedling development was delayed. This effect was eliminated by 25 mu mol l(-1) naproxen, an inhibitor of ABA biosynthesis. The Arabidopsis mutant deficient in ABA biosynthesis, nced3, displays a phenotype with more and longer LRs. We found that ABA decreased root-branching in peanut in a dose-dependent way. ABA-treated seedlings showed higher endogenous ABA levels than the control and naproxen-treated seedlings. RT-PCR results indicated that the expression of AhNCED1, a key gene in the ABA biosynthetic pathway, was significantly up-regulated by exogenous ABA in peanut. The mRNA levels of AhNCED1 began to increase 2 days after ABA treatment. The results from the current study show that ABA inhibits peanut LR development by increasing endogenous ABA contents.