Production of inflammatory mediators and cytokines by human gingival fibroblasts following bacterial challenge
Production of inflammatory mediators and cytokines by human gingival fibroblasts following bacterial challenge
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DOI：
10.1111/j.1600-0765.1996.tb00469.x
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发表时间：
1996-02-01
影响因子：
3.5
通讯作者：
Ebersole, JL
中科院分区：
文献类型：
--
作者：
DongariBagtzoglou, AI;Ebersole, JL
细菌通过激活宿主细胞产生和释放炎症介质和细胞因子，从而间接影响牙周病的病程。这些介质和细胞因子表现出强大的促炎和分解代谢活性，并可能在局部放大免疫反应以及牙周组织破裂中发挥关键作用。本研究检测了伴生放线杆菌(AA)和直肠弯曲菌(Cr)对人牙周成纤维细胞(HGF)产生PGF(2)、IL-1β、IL-6和IL-8的影响。制备接触抑制性肝细胞生长因子，加入10(6)~10(9)的福尔马林灭活细菌细胞(AAJP2、ATCC 29523&33384和CrATCC 33238)。以不同的时间间隔收集培养上清液，并分析细胞因子和介体含量。在最初的3-12小时内，所有浓度的AA、JP2和CRATCC 33238对IL-1β的产生都有大约50%的抑制作用。所测试的细菌浓度均不能使IL-1β的产量高于最大基础水平。这两种细菌都能刺激IL-6和IL-8的产生。AAJP2对PGE(2)水平无显著影响，而CrATCC 33238仅在所测试的最高浓度(10(9))下具有刺激作用。三株AA在IL-1β的产生方面没有显著差异。然而，AA ATCC 29523和ATCC 33384刺激IL-6分泌的能力弱于AA JP2，而刺激IL-8产生的能力强于AA JP2。总体而言，铬是HGF产生细胞因子和介质的最有效的促进剂。综上所述，AA和Cr可通过刺激HGF主要分泌IL-6和IL-8而增强局部免疫反应，促进牙周组织炎症反应。
Bacteria can indirectly affect the course of periodontal diseases by activating host cells to produce and release inflammatory mediators and cytokines. These mediators and cytokines manifest potent proinflammatory and catabolic activity and may play key roles in local amplification of the immune response as well as in periodontal tissue breakdown. This study tested the effect of Actinobacillus actinomycetemcomitans (Aa) and Campylobacter rectus (Cr) challenge on PGF(2) IL-1Beta, IL-6 and IL-8 production by human gingival fibroblasts (HGF). Contact-inhibited HGF were prepared and formalin-killed bacterial cells (Aa JP2, ATCC 29523 & 33384 and Cr ATCC 33238) at 10(6)-10(9) were added to the HGF. Culture supernatants were collected at varying time intervals and analyzed for cytokine and mediator content. All concentrations of Aa JP2 and CR ATCC 33238 suppressed IL-1Beta production up to approximately 50% during the initial 3-12h period. No bacterial concentration tested was able to increase IL-1Beta production above the maximum basal levels. Both bacterial species stimulated production of IL-6 and IL-8. Aa JP2 did not affect PGE(2) levels significantly, whereas Cr ATCC 33238 was stimulatory only at the highest concentration tested (10(9)). There were no significant differences among the three Aa strains with respect to IL-1BETA production. However, Aa ATCC 29523 and ATCC 33384 were less capable of stimulating IL-6 secretion and more efficient in stimulating IL-8 production than Aa JP2. In general, Cr was the most potent enhancer of cytokine and mediator production by HGF. In conclusion, Aa and Cr are capable of amplifying the local immune response and promoting periodontal tissue inflammation by stimulating HGF to secrete mainly IL-6 and IL-8.