Glycolytic flux in Saccharomyces cerevisiae is dependent on RNA polymerase III and its negative regulator Maf1
Glycolytic flux in Saccharomyces cerevisiae is dependent on RNA polymerase III and its negative regulator Maf1
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DOI：
10.1042/bcj20180701
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发表时间：
2018-07
影响因子：
4.1
通讯作者：
R. Szatkowska;Manuel Garcia-Albornoz;Katarzyna A. Roszkowska;S. Holman;E. Furmanek;S. Hubbard;R. Beynon;M. Adamczyk
中科院分区：
文献类型：
--
作者：
R. Szatkowska;Manuel Garcia-Albornoz;Katarzyna A. Roszkowska;S. Holman;E. Furmanek;S. Hubbard;R. Beynon;M. Adamczyk
蛋白质的生物合成在能量上是昂贵的，受到严格的调控，并与包括葡萄糖缺乏在内的应激条件有关。RNA聚合酶III(RNAP III)通过转录tDNA基因来合成tRNAs，是蛋白质生物合成的关键。在这里，我们分析了在富糖条件下RNAP III活性改变对酿酒酵母蛋白质组和代谢的影响。我们首次证明了RNAP III在分子系统水平上与糖酵解系统紧密耦合。当RNAP III活性降低或RNAP III负调控因子缺失时，Maf1会导致酿酒酵母中参与基本代谢的酶的丰度谱发生广泛的变化。在RNAP III活性受损的突变体中，以糖酵解能力为代价，重新分配到氨基酸合成从头开始。相反，缺乏Maf1蛋白的细胞具有更大的糖酵解通量潜力。
Protein biosynthesis is energetically costly, is tightly regulated and is coupled to stress conditions including glucose deprivation. RNA polymerase III (RNAP III) driven transcription of tDNA genes for production of tRNAs is a key element in efficient protein biosynthesis. Here we present an analysis of the effects of altered RNAP III activity on the Saccharomyces cerevisiae proteome and metabolism under glucose rich conditions. We show for the first time that RNAP III is tightly coupled to the glycolytic system at the molecular systems level. Decreased RNAP III activity or the absence of the RNAP III negative regulator, Maf1 elicit broad changes in the abundance profiles of enzymes engaged in fundamental metabolism in S. cerevisiae. In a mutant compromised in RNAP III activity there is a repartitioning towards amino acids synthesis de novo at the expense of glycolytic throughput. Conversely, cells lacking Maf1 protein have greater potential for glycolytic flux.