Hepatoma-derived growth factor is induced in liver regeneration
Hepatoma-derived growth factor is induced in liver regeneration
复制标题
DOI：
10.1111/j.1872-034x.2009.00532.x
复制
发表时间：
2009-10-01
影响因子：
4.2
通讯作者：
Nishiguchi, Shuhei
中科院分区：
文献类型：
--
作者：
Enomoto, Hirayuki;Nakamura, Hideji;Nishiguchi, Shuhei
目的：肝癌源性生长因子（HDGF）是一种肝素结合蛋白，参与肾脏、心血管系统和肝脏的发育。我们已经证明，HDGF在发育中的肝实质肝细胞中高度表达，并促进胎肝细胞增殖。本研究旨在探讨HDGF的表达与肝再生的关系。方法：在两种肝再生模型中检测HDGF的mRNA和蛋白表达。结果：在四氯化碳（CCl4）处理的再生肝中，HDGF表达被诱导，并在24 h达到高峰。HDGF在肝切除模型中表达也增强，并在术后12 h检测到峰值。蛋白质印迹法也证实了HDGF蛋白表达的增加。结论：HDGF在肝再生过程中的表达在肝实质细胞中占主导地位，在肝再生过程中，HDGF的表达先于肝实质细胞的DNA合成，提示HDGF可能作为一种自分泌因子参与肝再生。
Aim:Hepatoma-derived growth factor (HDGF) is a heparin-binding protein, which has been suggested to be involved in the development of kidneys, the cardiovascular system and the liver. We have shown that HDGF is highly expressed in parenchymal hepatocytes in the developing liver and promotes fetal hepatocyte proliferation. In the present study, we asked whether HDGF expression was related to liver regeneration.Methods:We examined the mRNA and protein expressions of HDGF in two liver regeneration models. In addition, cellular distribution of HDGF in the regenerating liver was investigated by immunohistochemistry.Results:In the carbon tetrachloride (CCl4)-treated liver, HDGF expression was induced and the peak was detected at 24 h after the CCl4 injection. HDGF expression was also enhanced in the hepatectomy model and the peak was detected at 12 h after surgery. The increased expression of HDGF protein was also confirmed by western blotting. Expression of the HDGF gene in the regenerating liver was dominantly detected in parenchymal hepatocytes.Conclusion:These findings showed that HDGF expression was induced in parenchymal hepatocytes before the DNA synthesis in the regenerating liver, suggesting the possible involvement of HDGF in liver regeneration as an autocrine factor.