LI-cadherin gene expression during mouse intestinal development
LI-cadherin gene expression during mouse intestinal development
复制标题
DOI：
10.1002/dvdy.1146
复制
发表时间：
2001-06-01
影响因子：
2.5
通讯作者：
Tauber, R
中科院分区：
文献类型：
--
作者：
Angres, B;Kim, L;Tauber, R
LI-钙粘蛋白（肝肠钙粘蛋白）是钙粘蛋白超家族中一个亚类（7-D 钙粘蛋白）的成员。尽管其作为细胞间粘附分子的细胞功能已在细胞培养研究中得到证实，但其生理功能仍需要在完整的生物体中进行探索。在分离小鼠LI-钙粘蛋白的cDNA后，我们产生了针对过表达蛋白的特异性抗体，并研究了其在成年小鼠组织和小鼠胚胎中的表达模式。小鼠 LI-钙粘蛋白序列与大鼠 LI-钙粘蛋白序列有 91% 相同，并表现出与大鼠 LI-钙粘蛋白相同的结构特征。在小鼠成体组织中，LI-钙粘蛋白在肠道中表达，并在脾脏中表达少量。与大鼠相比，小鼠LI-cadherin在肝脏中不表达，在小鼠胚胎发生过程中，LI-cadherin在胚胎第12.5天表达，除了在第13.5天在泌尿生殖窦和胆总管短暂表达外，LI-cadherin仅在肠上皮中发现，其表达与肠绒毛的形成相一致，这是一个包括主要组织重塑、生长和分化的发育阶段。 LI-钙粘蛋白沿发育中肠道的整个前后轴表达，一旦绒毛开始形成，则沿整个绒毛轴表达。 LI-cadherin占据上皮深层的所有细胞表面，仅在外上皮层细胞中分布于基底外侧表面，发现LI-cadherin总是与E-cadherin共表达。 (C) 2001 Wiley-Liss, Inc.
LI-cadherin (Liver-Intestine cadherin) is a member of a subclass (7-D cadherins) within the cadherin superfamily. Although its cellular function as a cell-cell adhesion molecule has been demonstrated in cell culture studies, its physiological function still needs to be explored in the intact organism, After isolating the cDNA for mouse LI-cadherin, we generated specific antibodies against the overexpressed protein and studied its expression pattern in adult mouse tissues and mouse embryos. The mouse LI-cadherin sequence is 91% identical to the sequence of rat LI-cadherin and exhibits the same structural features described for rat LI-cadherin, In mouse adult tissue, LI-cadherin is expressed in the intestine and in small amounts in the spleen. In contrast to rat, Mouse LI-cadherin was not expressed in liver, During mouse embryogenesis, LI-cadherin expression beans at embryonic day 12.5, With the exception of transient expression in the urogenital sinus and the common bile duct on day 13.5, LI-cadherin was found exclusively in the intestinal epithelium, Its expression coincides with the formation of intestinal villi, a developmental stage that includes major tissue remodeling, growth, and differentiation. LI-cadherin is expressed along the entire anterior-posterior axis of the developing intestine as well as along the entire villus axis once villi begin to form. LI-cadherin occupies all cell surfaces of the deeper layers of the epithelium, distributing to basolateral surfaces only in the cells of the outer epithelial layer, LI-cadherin was found to be always coexpressed with E-cadherin. (C) 2001 Wiley-Liss, Inc.