Quality evaluation of raw and processed Crataegi Fructus by color measurement and fingerprint analysis
Quality evaluation of raw and processed Crataegi Fructus by color measurement and fingerprint analysis
复制标题
通过比色和指纹图谱分析对生山楂和加工山楂进行质量评价。
DOI：
10.1002/jssc.201700575
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发表时间：
2018-01-01
影响因子：
3.1
通讯作者：
Yin, Fangzhou
中科院分区：
文献类型：
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作者：
Fei, Chenghao;Dai, Hui;Yin, Fangzhou
枸杞子及其炮制品作为传统药物使用已久，其疗效与众多的活性成分有关。然而，其加工产品的质量控制仍然缺乏一个全面和快速的方法。本研究建立了基于颜色测量和指纹图谱分析的两种分析方法。在颜色测量中，采用比色法对枸杞果皮和果肉的颜色值进行了评价。基于该结果，使用色度计建立颜色参考范围，并且使用中值颜色值建立标准色差值。采用贝叶斯线性判别分析法对枸杞及其炮制品的颜色值进行分析，建立预测枸杞炮制程度的数学函数。此外，在Hibar C18柱上进行高效液相色谱指纹图谱分析，并获得高效液相色谱指纹图谱，从中鉴定出9个峰。应用化学计量学方法成功地鉴别了枸杞子生品和炮制品。
Crataegi Fructus and its processed products have been used as a traditional medicine for a long time, and numerous active components are responsible for their curative effects. However, a comprehensive and fast method for the quality control of its processed products is still lacking. In this study, two analytical methods based on color measurements and fingerprint analysis are established. In the color measurements, the color values of the peel and flesh of Crataegi Fructus were evaluated spectrophotometrically. Based on the results, a color reference range was established using percentiles, and the standard color difference value was established using the median color values. Then, the color values of Crataegi Fructus and its processed products were analyzed using Bayes linear discriminant analysis and mathematical functions were built in order to predict the degree of processing. Moreover, high-performance liquid chromatography fingerprint analysis was performed on a Hibar C18 column, and a high-performance liquid chromatography fingerprint pattern was obtained, from which nine peaks were identified. Chemometric methods were successfully applied to differentiate raw and processed Crataegi Fructus.