Validation and the Determination of Antibody Bioactivity Using MILKSHAKE and Sundae Protocols.
Validation and the Determination of Antibody Bioactivity Using MILKSHAKE and Sundae Protocols.
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使用奶昔和圣代方案验证和测定抗体生物活性。
DOI：
10.1007/978-1-0716-3381-6_24
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Li,Xiaofeng
中科院分区：
文献类型：
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作者：
Ferguson,MaryR;Mendez,QianaM;Acca,FelicityE;Chapados,CassandraD;Driscoll,HollandA;Jones,KezziaS;Mirando,Gregory;Weiner,MichaelP;Li,Xiaofeng
为了获得可重现的结果，实验中使用的所有试剂必须以替代或独立的方法进行验证。我们提出了两种用于确定抗体特异性的独立方法：（1）“MILKSHAKE”，其可用于验证针对后修饰表位的抗体的可靠性和特异性，以及（2）“Sundae”，其是一种更完整的丙氨酸样扫描方法，其可用于更好地理解蛋白质的特定残基的结合和生物活性。我们将这两种方法应用于抗体与其抗原之间的相互作用。
To develop reproducible results, it is critical that all reagents used in an experiment be validated in an alternative or independent method. We present two such independent methods for determining the specificity of antibodies: (1) “MILKSHAKE,” which can be used to validate the liability and specificity of antibodies directed against post-translationally-modified epitopes, and (2) “Sundae,” which is a more complete alanine-like scanning method that can be used to better understand the binding and bioactivity of specific residues of a protein. We apply both of these methods to the interaction between an antibody and its antigen.