A review on optical imaging of DNA nanostructures and dynamic processes
A review on optical imaging of DNA nanostructures and dynamic processes
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DOI：
10.1088/2050-6120/aaed11
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发表时间：
2019-01
影响因子：
3.2
通讯作者：
Yancheng Du;Jing Pan;J. Choi
中科院分区：
文献类型：
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作者：
Yancheng Du;Jing Pan;J. Choi
DNA自组装为构建复杂的纳米结构和编程动态分子过程（如链位移）提供了强有力的手段。DNA纳米系统在小尺度（通常小于100纳米）内具有很高的结构复杂性，并且在长时间内具有动态特征，这给表征带来了新的挑战。DNA纳米系统的时空特征需要新颖的实验方法，能够在长时间内进行高分辨率成像。本文综述了表征自组装DNA纳米系统的光学成像方法的最新进展，特别强调了超分辨荧光显微镜。开发了几种先进的策略来获得复杂的DNA纳米几何形状的准确和详细的图像，并在动态过程中执行分子运动的精确跟踪。我们介绍了最先进的仪器和成像策略，包括定位显微镜和光谱成像。我们讨论了它们在生物学研究和生物医学应用中的应用，并提供了当前的挑战和未来的展望。综上所述，本文为DNA纳米技术领域的光学显微镜研究提供了实用指南。
DNA self-assembly offers a powerful means to construct complex nanostructures and program dynamic molecular processes such as strand displacement. DNA nanosystems pack high structural complexity in a small scale (typically, <100 nm) and span dynamic features over long periods of time, which bring new challenges for characterizations. The spatial and temporal features of DNA nanosystems require novel experimental methods capable of high resolution imaging over long time periods. This article reviews recent advances in optical imaging methods for characterizing self-assembled DNA nanosystems, with particular emphasis on super-resolved fluorescence microscopy. Several advanced strategies are developed to obtain accurate and detailed images of intricate DNA nanogeometries and to perform precise tracking of molecular motions in dynamic processes. We present state-of-the-art instruments and imaging strategies including localization microscopy and spectral imaging. We discuss how they are used in biological studies and biomedical applications, and also provide current challenges and future outlook. Overall, this review serves as a practical guide in optical microscopy for the field of DNA nanotechnology.