Expression of microRNA in follicular fluid in women with and without PCOS
Expression of microRNA in follicular fluid in women with and without PCOS
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DOI：
10.1038/s41598-019-52856-5
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发表时间：
2019-11-08
影响因子：
4.6
通讯作者：
Atkin, Stephen L.
中科院分区：
文献类型：
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作者：
Butler, Alexandra E.;Ramachandran, Vimal;Atkin, Stephen L.
一些研究表明，小的非编码microRNA(MiRNA)在PCOS中的表达及其在卵泡液中的表达已被描述，尽管研究的数量仍然很少。在这项前瞻性队列研究中，用定量聚合酶链式反应(QPCR)检测了29名体重和年龄匹配的无排卵多囊卵巢综合征妇女和30名未取卵泡液的体外受精(IVF)妇女的miRNA水平，并根据miRNA数据集确定miRNA水平。共检测到176个miRNA，其中29个miRNA在正常妇女和多囊卵巢综合征妇女之间存在显著差异。其中前7位(p<；0.015)为miR-381-3p、miR-199B-5p、miR-93-3p、miR-361-3p、miR-127-3p、miR-382-5p、miR-425-3p。在PCOS中，miR-382-5p与年龄、游离雄激素指数(FAI)相关，miR-199B-5p与抗髓鞘激素(AMH)相关，miR-93-3p与C反应蛋白(CRP)相关。正常对照组miR-127-3p、miR-382-5p、miR-425-3p与受精率相关，miR-127-3p与胰岛素抵抗相关，miR-381-3p与FAI相关。独创性途径评估显示，12个显著改变的miRNA与生殖途径有关，12个miRNA与炎症性疾病途径有关，6个与良性盆腔疾病有关。根据独创性路径分析，PCOS患者卵泡液中miRNAs的表达与年龄、FAI、炎症和AMH有关，与PCOS患者的BMI、受精率(3miRNA)、胰岛素抵抗、FAI和炎症有关。
Several studies have shown the expression of small non-coding microRNA (miRNA) changes in PCOS and their expression in follicular fluid has been described, though the number of studies remains small. In this prospective cohort study, miRNA were measured using quantitative polymerase chain reaction (qPCR) in 29 weight and aged matched anovulatory women with PCOS and 30 women without from follicular fluid taken at the time of oocyte retrieval who were undergoing in vitro fertilization (IVF); miRNA levels were determined from a miRNA data set. 176 miRNA were detected, of which 29 differed significantly between normal women and PCOS women. Of these, the top 7 (p < 0.015) were miR-381-3p, miR-199b-5p, miR-93-3p, miR-361-3p, miR-127-3p, miR-382-5p, miR-425-3p. In PCOS, miR-382-5p correlated with age and free androgen index (FAI), miR-199b-5p correlated with antimullerian hormone (AMH) and miR-93-3p correlated with C-reactive protein (CRP). In normal controls, miR-127-3p, miR-382-5p and miR-425-3p correlated with the fertilisation rate; miR-127-3p correlated with insulin resistance and miR-381-3p correlated with FAI. Ingenuity pathway assessment revealed that 12 of the significantly altered miRNA related to reproductive pathways, 12 miRNA related to the inflammatory disease pathway and 6 were implicated in benign pelvic disease. MiRNAs differed in the follicular fluid between PCOS and normal control women, correlating with age, FAI, inflammation and AMH in PCOS, and with BMI, fertilization rate (3 miRNA), insulin resistance, FAI and inflammation in control women, according to Ingenuity Pathway Analysis.