CLONING, SEQUENCING AND PRODUCTION OF THE LANTIBIOTIC MERSACIDIN
CLONING, SEQUENCING AND PRODUCTION OF THE LANTIBIOTIC MERSACIDIN
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DOI：
10.1016/0378-1097(95)00048-a
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发表时间：
1995-03-15
影响因子：
2.1
通讯作者：
SAHL, HG
中科院分区：
文献类型：
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作者：
BIERBAUM, G;BROTZ, H;SAHL, HG
Mersacidin是一种含硫肽的多肽抗生素，对耐甲氧西林金黄色葡萄球菌具有良好的体内药效。它在固定期早期被排出体外，用反相高效液相色谱法一步从培养上清中纯化。其结构基因从枯草芽孢杆菌HIL y -85,54728的染色体DNA中克隆得到。测序结果显示，mersacidin由一个异常长的48个氨基酸的先导序列和一个20个氨基酸的前肽部分组成，该前肽部分在生物合成过程中被修饰为成熟的抗生素。mersacidin前肽与已知抗生素的比较表明，mersacidin与B型抗生素肉桂霉素的关系比与A型抗生素的关系更密切。
Mersacidin is a lanthionine-containing peptide antibiotic that shows a good in vivo efficiency against methicillin-resistant Staphylococcus aureus. It is excreted during early stationary phase and could be purified from culture supernatant in a one-step procedure by reversed phase HPLC. Its structural gene was cloned from chromosomal DNA of the producer strain Bacillus subtilis HIL Y-85,54728. Sequencing revealed that pre-mersacidin consists of an unusually long 48 amino acid leader sequence and a 20 amino acid propeptide part which is modified during biosynthesis to the mature lantibiotic. The comparison of the mersacidin prepeptide with those of hitherto known lantibiotics demonstrates that mersacidin is more closely related to type B lantibiotic cinnamycin than to type A antibiotics.