EVIDENCE FOR ADRENALIN NEURONS IN RAT-BRAIN
EVIDENCE FOR ADRENALIN NEURONS IN RAT-BRAIN
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DOI：
10.1111/j.1748-1716.1973.tb05522.x
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发表时间：
1973-01-01
期刊：
影响因子：
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通讯作者：
JOHANSSON, O
中科院分区：
文献类型：
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作者：
HOKFELT, T;FUXE, K;JOHANSSON, O
. 2clt.依那林具有1）EC。在生物化学研究中发现在大鼠脑中存在N。[1954]，Gunne 1962）。更进一步。第01章. j 1969）和Pohorecky ct NI.在1969年，我能够证明在1）txiii的肾上腺素合成。尽管事实上肾上腺素主要来自于心脏，但仍然有一场骚乱。用甲醛荧光法（Falck-Hillarp）对动物脑内的肾上腺皮质神经元进行组织化学观察，结果未见异常，可能是由于该方法在区分不同种类的儿茶酚胺类神经元方面存在局限性。将免疫组织化学引入铁转运体研究（kiien ct（11。1969年），开辟了新的可能性，以确定atid本地nionoartiinc rieurotis。在本研究中，我们使用了苯乙醇胺-N-甲基转移酶（PNMT）的抗体。去甲肾上腺素和肾上腺素的反应本文用间接免疫荧光技术追踪了该药物在大鼠海恩中的可能细胞定位。在与PNMT的抗血清孵育，然后与异硫氰酸荧光素抗体孵育后，获得某些神经炎的特异性染色。~（311）Bor1ic. R分布于两组延髓：ITA。第一个（C1组）位于延髓头端外侧的网状结构，位于第11核的尾侧，橄榄线圈的外侧。farialis（图1）。这种分布与群吻部的嗜铬细胞体非常相似。41.第二细胞群（C2群）也出现在延脑吻侧水平，主要围绕第四脑室腹面内侧部。Vt~ rz~ tr. rnriiici1.在脑stetti的v、iriotrs核中发现q。经常在课外活动区。在核内发现了一个由PNM 'I'阳性神经终末组成的denye网络。背侧耳轴11.迷走神经（图2），神经束核。孤束核、室旁核、丘脑丘状细胞核、室旁核和脊髓外侧交感核。密度适中，
. 2clt. enaline has 1) ec. n found in thc rat brain in biocheniical investigation.; j I’ogt 1954, Gunne 1962). Furthertriore. Ciarancllo r’t 01. j 1969) and Pohorecky ct NI. I I969 i were able to denimstrate etizymatic adrenaline synthesis in the 1) txiii of\. a ri ot is niaiiitiin Is.I n spite of the fact that aclrcnaline principally cmi he\. isualized with the forriinl-dehyde fluorescence j Falck-Hillarp) technique no histochemical rviderice ha5 so far heen preyented for adrcnalirie neurons in the inaninialian brain, probably due to tliifictilties in differentiating bctween various catecholamines with this itlethod. The introduction of iminunohistocheniistry into tieurotransniitter research ((kiien ct (11. 1969) has opened up new possibilities to idcntify atid localize nionoartiinc rieurotis. In the present study we have used antibodics to phenylethanolatnirie-N-methyltransferase (PNMT). the enxyrtie cotivcriing noradrenaline to adrerialine. in; in attempt to trace the possililc cellular localization of this erizyine in the rat hain with the indirect iniruunofluorescence technique.. A specific staining of certain neurotis was obtained after incubation with anti-serutn to PNMT followed by incubation with flttorescciti isothiocyanate corijugatrd nntibodies. The~(311 bor1ic. r were localized iii two groups in the medulla obloiig: ita.’l’he first onc (Group C1) was localized in the\witrolateral rcticular formation oi the rostl-nl ttiedulla oblongata laterally of the olivary coiriples arid caudal to the nucleus 11. farialis (Fig. 1). This distribution is very similar to the cxtecholaniine cell bodic~ s in the rostral part of group. 41. The second cell group (Croup C2) was also found at the rostral level of the medulla oblongata niainly surrounding the medial part of tlre Lrentral surface of the fourth ventricle.. Vt~ rz~~~ tr. rnriiici1. q were found in v; iriotrs nuclei of the brain stetti. often in perivctiitricular areas. A denye network of PNM ‘I’positive ncrve terminals were found in tlie nuc! eus niotorius dorsalis 11. vagi (Fig. 2), nucleus tr. solitarii, nucleus paravcntric1iI; tris tllagrlocellularis, nucleus paraventricularis rottindocellularis thalami, and also in the lateral sympathetic riucleus of the spinal cord. A moderate density was ob-