Endocannabinoid modulation of sympathetic and cardiovascular responses to acute stress in the periaqueductal gray of the rat
Endocannabinoid modulation of sympathetic and cardiovascular responses to acute stress in the periaqueductal gray of the rat
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DOI：
10.1152/ajpregu.00391.2010
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发表时间：
2011-03-01
影响因子：
2.8
通讯作者：
Dean, C.
中科院分区：
文献类型：
--
作者：
Dean, C.
交感神经系统的激活是对压力或危险作出协调反应的基础。中脑导水管周围灰质（PAG）包含招募交感神经系统和组织生理和行为反应所需的神经基质，以应对强加的挑战。内源性大麻素已被证明能影响相关的行为反应。本研究使用防御反应作为工作模型来研究内源性大麻素对麻醉大鼠急性应激交感反应的调节。将大麻素1 （CB1）受体激动剂anandamide微注射到PAG背侧防御通路，可引起肾交感神经活动和血压升高，触须抽搐和肢体运动。事先在同一部位注射CB1受体拮抗剂AM-281可减弱该反应。电刺激下丘脑防御区可以引起类似的交感兴奋和压力反应，在PAG背侧微量注射AM-281后，这些反应明显减弱。这些数据表明，内源性大麻素可以在PAG水平上通过CB1受体调节急性应激反应的交感和心血管成分。
Activation of the sympathetic nervous system is fundamental to the coordinated response to stress or danger. The midbrain periaqueductal gray (PAG) contains the neural substrate required to recruit the sympathetic nervous system and organize the physiological and behavioral responses required to respond to imposed challenges. Endocannabinoids have been shown to influence associated behavioral responses. The defense response was used in this study as a working model to examine endocannabinoid modulation of the sympathetic response to acute stress in the anesthetized rat. Microinjection of the cannabinoid 1 (CB1) receptor agonist anandamide into the defense pathway of the dorsal PAG could elicit an increase in renal sympathetic nerve activity and blood pressure, twitching of the whiskers, and movement of the limbs. The response was attenuated by prior microinjection of the CB1 receptor antagonist AM-281 at the same site. Electrical stimulation of the hypothalamic defense area could evoke similar sympathoexcitatory and pressor responses, which were significantly attenuated by microinjection of AM-281 into the dorsal PAG. These data indicate that endocannabinoids can modulate the sympathetic and cardiovascular components of the acute stress response via CB1 receptors at the level of the PAG.