Effects of ROS and caspase-3-like protein on the growth and aerenchyma formation of Potamogeton perfoliatus stem
Effects of ROS and caspase-3-like protein on the growth and aerenchyma formation of Potamogeton perfoliatus stem
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DOI：
10.1007/s00709-022-01780-z
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发表时间：
2022-06
期刊：
影响因子：
2.9
通讯作者：
Qi Xie;Zhongxun Yuan;Hui Hou;Honglian Zhao;Hao Chen;Xilu Ni
中科院分区：
文献类型：
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作者：
Qi Xie;Zhongxun Yuan;Hui Hou;Honglian Zhao;Hao Chen;Xilu Ni
通气组织的形成对透叶马铃薯在水下环境中的生存起着重要的作用。为了了解活性氧（ROS）和caspase-3样蛋白信号分子在通气组织形成中的调节作用，我们研究了外源性NADPH氧化酶抑制剂（二苯二氯铵，DPI）、过氧化氢酶抑制剂（3-氨基-1,2,4-三唑，AT）和caspase-3样蛋白抑制剂（AC-DEVD-CHO， DEVD）对p形态学和生理特征以及通气组织形成的影响。perfoliatus。结果表明，DPI处理后，caspase-3样蛋白活性降低，ros相关酶活性升高，h2o2含量降低，从而抑制了通气组织的形成。当DPI浓度约为1 μmol/L时，抑制效果最明显。相反，经AT处理后，caspase-3样蛋白活性升高，ros相关酶活性降低，h2o2含量增加，最终促进了气孔的形成，且在约500 μmol/L AT处理下，促进作用最为明显。经DEVD处理后，DPI或AT处理对营养生长的抑制得到缓解，caspase-3样活性明显降低，通气组织发育受到抑制。本研究结果表明，ROS对通气组织形成具有正向调节作用，激活caspase-3样蛋白促进ROS介导的通气组织形成。本实验为进一步探索ROS和caspase-3样蛋白在植物细胞中的信号转导作用及其在植物发育中的作用提供了新的理论基础。
Aerenchyma formation plays an important role in the survival ofPotamogeton perfoliatusin submerged environment. To understand the regulatory role of reactive oxygen species (ROS) and caspase 3-like protein signaling molecules in aerenchyma formation, we investigated the effects of exogenous NADPH oxidase inhibitor (diphenyleneiodonium chloride, DPI), catalase inhibitor (3-amino-1,2,4-triazole, AT), and caspase-3-like protein inhibitor (AC-DEVD-CHO, DEVD) on morphological and physiological characteristics and aerenchyma formation inP. perfoliatus. The results showed that after DPI treatment, caspase-3-like protein activity decreased, ROS-related enzyme activities increased, and H2O2content decreased, thereby inhibiting aerenchyma formation. When the concentration of DPI was approximately 1 μmol/L, the inhibitory effect was the most obvious. On the contrary, after the AT treatment, caspase-3-like protein activity increased, ROS-related enzyme activities decreased, and the H2O2content increased, ultimately promoting aerenchyma formation, and the promotion was the most obvious under treatment with approximately 500 μmol/L AT. After DEVD treatment, the inhibition of vegetative growth caused by DPI or AT treatment was alleviated, significantly reducing caspase-3-like activity and inhibiting aerenchyma development. The results of this study show that ROS has a positive regulatory effect on aerenchyma formation, and caspase-3-like protein is activated to promote ROS-mediated aerenchyma formation. This experiment provides a new theoretical basis for further exploration of the signal transduction effects of ROS and caspase-3-like protein in plant cells and their roles in plant development.