Isolation of cDNA encoding a binding protein specific to 5'-phosphorylated single-stranded DNA with G-rich sequences.
Isolation of cDNA encoding a binding protein specific to 5'-phosphorylated single-stranded DNA with G-rich sequences.
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分离编码具有富含 G 序列的 5-磷酸化单链 DNA 特异性结合蛋白的 cDNA。
DOI：
10.1093/nar/21.8.1761
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发表时间：
1993
影响因子：
14.9
通讯作者：
T. Honjo
中科院分区：
文献类型：
--
作者：
T. Mizuta;Y. Fukita;T. Miyoshi;A. Shimizu;T. Honjo
我们已经分离出的cDNA编码的结合蛋白的序列基序的免疫球蛋白S亩区的西南方法。命名为S μ bp-2的结合蛋白特异性结合含有5 ′-G和GGGG段的5 ′-磷酸化单链DNA。从cDNA序列推导的氨基酸序列表明，S mu bp-2属于推定的解旋酶超家族，参与复制，重组和修复。S μ bp-2 mRNA的表达是普遍存在的，并在用脂多糖和白细胞介素4刺激的脾细胞中增加，这也诱导类转换。Smu bp-2基因在脊椎动物中是保守的。可能参与的S亩bp-2类切换进行了讨论。
We have isolated the cDNA encoding a binding protein to the sequence motif of the immunoglobulin S mu region by the southwestern method. The binding protein designated S mu bp-2 specifically binds to 5'-phosphorylated single-stranded DNA containing 5'-G and GGGG stretches. The amino acid sequence deduced from the cDNA sequence showed that the S mu bp-2 belongs to the putative helicase superfamily which is involved in replication, recombination and repair. Expression of S mu bp-2 mRNA is ubiquitous and augmented in spleen cells stimulated with lipopolysaccharide and interleukin 4 which also induce class switching. The S mu bp-2 gene is conserved among vertebrates. Possible involvement of S mu bp-2 in class switching is discussed.