Initiation of runaway cell death in an Arabidopsis mutant by extracellular superoxide
Initiation of runaway cell death in an Arabidopsis mutant by extracellular superoxide
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DOI：
10.1126/science.273.5283.1853
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发表时间：
1996-09-27
期刊：
影响因子：
56.9
通讯作者：
Dangl, JL
中科院分区：
文献类型：
--
作者：
Jabs, T;Dietrich, RA;Dangl, JL
活性氧中间体（ROIs）在动物正常发育和疾病过程中调节细胞凋亡。roi还与植物对病原体的超敏抗性反应有关。拟南芥Isd1突变体在没有病原体的情况下对细胞死亡的控制受损，一旦细胞死亡开始就不能控制细胞死亡的传播。超氧化物是启动病变形成的必要和充分条件；它在细胞死亡前积累，随后在扩散的Isd1病变附近的活细胞中积累。因此，在Isd1植物中发现的失控细胞死亡反映了超氧化物的异常积累和对其产生的信号缺乏反应。
Reactive oxygen intermediates (ROIs) regulate apoptosis during normal development and disease in animals. ROIs are also implicated in hypersensitive resistance responses of plants against pathogens. Arabidopsis Isd1 mutants exhibited impaired control of cell death in the absence of pathogen and could not control the spread of cell death once it was initiated. Superoxide was necessary and sufficient to initiate lesion formation; it accumulated before the onset of cell death and subsequently in live cells adjacent to spreading Isd1 lesions, Thus, runaway cell death seen in Isd1 plants reflected abnormal accumulation of superoxide and lack of responsiveness to signals derived from it.