Isolation and identification of Tete virus group (Peribunyaviridae: Orthobunyavirus) from Culicoides biting midges collected in Lichuan County, China.
Isolation and identification of Tete virus group (Peribunyaviridae: Orthobunyavirus) from Culicoides biting midges collected in Lichuan County, China.
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DOI：
10.3389/fcimb.2023.1193184
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发表时间：
2023
影响因子：
5.7
通讯作者：
中科院分区：
文献类型：
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作者：
2018年7月，从江西省利川县采集的库蠓中分离到1株病毒（JXLC1806-2）。该病毒分离物在接种哺乳动物细胞（BHK-21）后48小时内表现出明显的细胞病变作用。JXLC1806-2病毒可在BHK-21细胞内形成斑块，病毒滴度为1×105.6 pfu/mL。接种病毒后，乳鼠发病死亡。核苷酸和氨基酸序列分析表明，JXLC1806-2病毒基因组由S、M和L三段组成。系统发育分析表明，JXLC1806-2病毒的S、M和L基因属于太特病毒（Tete）血清群正布尼亚病毒（Orthobunyavirus），但与太特病毒（Tete）血清群的其他成员形成独立的进化分支。结果表明，JXLC1806-2病毒为太特血清群的新成员，命名为利川病毒，这是中国首次分离到太特血清群病毒。
In July 2018, a virus (JXLC1806-2) was isolated from Culicoides biting midges collected in Lichuan County, Jiangxi Province, China. The virus isolate showed significant cytopathic effects within 48 hours after inoculation with mammalian cells (BHK-21). JXLC1806-2 virus could form plaques in BHK-21 cells, and the virus titer was 1×105.6 pfu/mL. After inoculation with the virus, suckling mice developed disease and died. The nucleotide and amino sequence analysis showed that the JXLC1806-2 virus genome was composed of S, M and L segments. Phylogenetic analysis showed that the S, M and L genes of JXLC1806-2 virus belonged to the Tete serogroup, Orthobunyavirus, but formed an independent evolutionary branch from the other members of the Tete serogroup. The results showed that the JXLC1806-2 virus, which was named as Lichuan virus, is a new member of Tete serogroup, and this is the first time that a Tete serogroup virus has been isolated in China.