Acquisition of Rickettsia felis by cat fleas during feeding.
Acquisition of Rickettsia felis by cat fleas during feeding.
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猫蚤在进食过程中感染猫立克次体。
DOI：
10.1089/vbz.2010.0137
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Macaluso,KevinR
中科院分区：
文献类型：
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作者：
Reif,KathrynE;Kearney,MichaelT;Foil,LaneD;Macaluso,KevinR
费利立克次体水平传播途径的证据来自于 R 的检测。脊椎动物和多种吸血节肢动物的猫感染；然而，尚未证实在吸血期间感染猫蚤（Ctenocephalides felis）。在这项研究中，猫蚤获得R的能力。猫科动物通过传染性血粉和随后的垂直传播进行了检查。利用人工饲养系统，将未接触立克次体的跳蚤暴露于R.猫科动物感染的血液并每周监测一次感染情况，持续 4 周。暴露后 7 天 (dpe)，约 52% 的跳蚤成功感染立克次体和立克次体。猫DNA；在猫跳蚤粪便中检测到立克次体转录本和 DNA。实时定量聚合酶链反应确定 R.猫感染负荷和R。在稍后的时间点评估的跳蚤中，猫感染密度显着更高。虽然是执着的R。在成年跳蚤中检测到猫感染，R.在 F1 后代中未观察到猫感染。这项研究表明猫蚤能够获得 R。来自传染性血粉的猫感染，并将作为检查 R 的模型。猫科动物在节肢动物和脊椎动物宿主之间传播。
Evidence for horizontal routes of transmission forRickettsia felishas come from detection ofR. felisinfection in vertebrates and multiple blood-feeding arthropods; however, infection of cat fleas,Ctenocephalides felis, during blood feeding has not been demonstrated. In this study, the ability of cat fleas to acquireR. felisthrough an infectious blood meal with subsequent vertical transmission was examined. Utilizing an artificial feeding system,Rickettsia-naive fleas were exposed toR. felis–infected blood meals and monitored for subsequent infection at weekly intervals for 4 weeks. At 7 days postexposure (dpe) ∼52% of fleas successfully acquired rickettsiae andR. felisDNA; rickettsial transcript and DNA was detected in cat flea feces. Quantitative real-time polymerase chain reaction determined that both theR. felisinfection load andR. felisinfection density was significantly greater in fleas assessed at later time points. Although a persistentR. felisinfection was detected in adult fleas,R. felisinfection was not observed in F1progeny. This study demonstrates that cat fleas are able to acquireR. felisinfection from an infectious blood meal and will serve as a model to examineR. felistransmission between arthropod and vertebrate hosts.