Diverse cellular localizations of secretory phospholipase A2 enzymes in several human tissues
Diverse cellular localizations of secretory phospholipase A2 enzymes in several human tissues
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DOI：
10.1016/j.bbalip.2005.08.014
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发表时间：
2005-10-01
影响因子：
4.8
通讯作者：
Kudo, I
中科院分区：
文献类型：
--
作者：
Masuda, S;Murakami, M;Kudo, I
哺乳动物分泌型磷脂酶A（2）（sPLA（2））家族包含10多种酶。在这项研究中，我们通过免疫组织化学方法检测了六种sPLA（2）（IIA，IID，IIE，IIF，V和X）在人心脏，肾脏，肝脏和胃中的定位。在正常心脏中，sPLA（2）-IIA在冠状动脉血管平滑肌细胞（VSMC）中被检测到，sPLA（2）-V在内膜下的心肌细胞中被检测到。在梗死的心脏中，这两种酶的表达在受损的心肌细胞中显著增加，sPLA（2）-IID和-IIE的表达在正常心脏中检测不到，在受损的心肌细胞和VSMC中分别升高。在梗死肾脏中，sPLA（2）-IIA和-V在肾小管上皮细胞中显著诱导。在受病毒性肝炎影响的肝脏中，sPLA（2）-IIA和-V在脂肪变性的肝细胞中表达。在肠上皮化生的胃腺中，sPLA（2）-IIA定位于腺基部，sPLA（2）-IID和-V定位于腺体上皮，sPLA（2）-IIE和-IIF定位于小窝上皮的杯状细胞，sPLA（2）-X定位于腺体上皮细胞和小窝上皮杯状细胞。在胃粘膜下组织中，sPLA（2）-IIA和-IIE定位于VSMC，sPLA（2）-V定位于间质成纤维细胞。sPLA（2）-IIA、-IIE、-IIF和-X在胃印戒细胞癌中高表达。因此，单个sPLA（2）在每个组织中表现出独特的细胞定位，表明它们在病理生理学中的独特作用。(c)2005 Elsevier B. V.保留所有权利。
The secretory phospholipase A(2) (sPLA(2)) family in mammals contains more than 10 enzymes. In this study, we examined by immunohistochemistry the localization of six sPLA(2)s (IIA, IID, IIE, IIF, V and X) in human heart, kidney, liver and stomach. In normal hearts, sPLA(2)-IIA was detected in coronary vascular smooth muscle cells (VSMC) and sPLA(2)-V in cardiomyocytes beneath the endocardium. In infarcted hearts, expression of these two enzymes was markedly increased in damaged cardiomyocytes, and expression of sPLA(2)-IID and-IIE, which was undetectable in normal hearts, was elevated in damaged cardiomyocytes and VSMC, respectively. In infarcted kidneys, sPLA(2)-IIA and-V were markedly induced in the uriniferous tubular epithelium. In livers affected by viral hepatitis, sPLA(2)-IIA and-V were expressed in hepatocytes with fatty degeneration. In the gastric glands exhibiting intestinal metaplasia, sPLA(2)-IIA was localized in the glandular base, sPLA(2)-IID and-V in the glandular body epithelium, sPLA(2)-IIE and-IIF in goblet cells in the foveolar epithelium, and sPLA(2)-X in both glandular body epithelial cells and foveolar epithelial goblet cells. In the gastric submucosal tissues, sPLA(2)-IIA and-IIE were located in VSMC and sPLA(2)-V was in the interstitial fibroblasts. In addition, sPLA(2)-IIA,-IIE,-IIF and-X were highly expressed in gastric signet ring cell carcinoma. Thus, individual sPLA(2)s exhibit unique cellular localizations in each tissue, suggesting their distinct roles in pathophysiology. (c) 2005 Elsevier B.V. All rights reserved.