DNA STRAND SCISSION BY ANTITUMOR PROTEIN NEOCARZINOSTATIN
DNA STRAND SCISSION BY ANTITUMOR PROTEIN NEOCARZINOSTATIN
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DOI：
10.1016/0006-291x(74)90449-5
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发表时间：
1974-01-01
影响因子：
3.1
通讯作者：
GOLDBERG, IH
中科院分区：
文献类型：
--
作者：
BEERMAN, TA;GOLDBERG, IH
抗生素蛋白，新抑癌素，在体内和体外诱导DNA链的断裂。当细胞与0.5 - 5.0 μg/ml高度纯化的新制癌素孵育时，用[14 C]胸苷预标记的HeLa细胞DNA被切成在80- 90 S处具有峰的大块。在2-巯基乙醇存在下，将抗生素（0.5 μg/ml）与[3 H] SV 40 DNA孵育，导致超螺旋DNA I转化为带切口的环状双链DNA II。在高水平的药物下，产生较小的线性DNA片段。在中性和碱性蔗糖梯度中都检测到链断裂，表明药物敏感性不是由于碱不稳定键。
The antibiotic protein, neocarzinostatin, induces the scission of DNA strands in vivo and in vitro. HeLa cell DNA prelabelled with [14 C] thymidine is cut into large pieces with a peak at 80–90S when cells are incubated with 0.5 to 5.0 μg/ml of highly purified neocarzinostatin. Incubation of the antibiotic (0.5 μg/ml) with [3 H] SV40 DNA in the presence of 2-mercaptoethanol results in the conversion of superhelical DNA I to nicked circular duplex DNA II. At high levels of drug, smaller fragments of linear DNA are produced. Strand breaks are detected in both neutral and alkaline sucrose gradients, indicating that drug susceptibility is not due to alkali-labile bonds.