Investigation of killer cell immunoglobulin-like receptor gene diversity in KIR3DL1 and KIR3DS1 in a transplant population
Investigation of killer cell immunoglobulin-like receptor gene diversity in KIR3DL1 and KIR3DS1 in a transplant population
复制标题
DOI：
10.1111/j.1399-0039.2008.01017.x
复制
发表时间：
2008-05-01
期刊：
影响因子：
--
通讯作者：
Hurley, C. K.
中科院分区：
文献类型：
--
作者：
Belle, I.;Hou, L.;Hurley, C. K.
用几个重叠扩增子从一个家族和77名骨髓移植患者及其无关供体中获得覆盖KIR3DL1和KIR3DS1大部分编码区的基因组DNA序列。等位基因3DL1*00101和 *002最常见，除了12个其他已知的3DL1等位基因。一个单一的3DS1等位基因，3DS1*01301，在32个携带该基因的个体中的31个中被鉴定。发现两个新的等位基因3DL1*01702和3DS1*058。三个样本似乎携带重复的杀伤细胞免疫球蛋白样受体（KIR）单倍型在其他研究中观察到的基础上存在的3DS1和两个3DL1等位基因。此外，一个样本似乎携带一种新的KIR单倍型，含有一个3DL1和两个3DS1等位基因。
Several overlapping amplicons were used to obtain the sequence of genomic DNA covering most of the coding regions of KIR3DL1 and KIR3DS1 from a family and 77 bone marrow transplant patients and their unrelated donors. Alleles 3DL1*00101 and *002 were most frequently observed in addition to 12 other known 3DL1 alleles. A single 3DS1 allele, 3DS1*01301, was identified in the 31 of 32 individuals carrying this gene. Two new alleles, 3DL1*01702 and 3DS1*058, were characterized. Three samples appeared to carry the duplicated killer cell immunoglobulin-like receptor (KIR) haplotype observed in other studies based on the presence of 3DS1 and two 3DL1 alleles. Additionally, one sample appeared to carry a novel KIR haplotype containing one 3DL1 and two 3DS1 alleles.