Complete RNAi rescue of neuronal degeneration in a constitutively active Drosophila TRP channel mutant
Complete RNAi rescue of neuronal degeneration in a constitutively active Drosophila TRP channel mutant
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DOI：
10.1016/j.bbagen.2004.06.002
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发表时间：
2004-09-06
影响因子：
3
通讯作者：
Pak, WL
中科院分区：
文献类型：
--
作者：
Geng, CX;Pellegrino, A;Pak, WL
RNA干扰已被广泛用于减少目标基因编码的蛋白质数量。色氨酸（P365）是色氨酸的一种组成性的、活跃的显性等位基因，它通过持续和过量的Ca2+内流导致光感受器的大量变性。在这里，我们表明RNAi在TRP （P365）杂合子中大量减少TRP通道蛋白完全挽救了神经元变性，并显着改善了眼睛的光激发反应。这种减少不一定是完全的，这表明挽救变性是可能的，并且由于靶蛋白的过度消耗而产生的副作用最小。(C) 2004 Elsevier B.V.版权所有
RNA interference has been widely used to reduce the quantity of the proteins encoded by the targeted genes. A constitutively active, dominant allele of trp, Trp(P365), causes massive degeneration of photoreceptors through a persistent and excessive Ca2+ influx. Here we show that a substantial reduction of the TRP channel protein by RNAi in Trp(P365) heterozygotes completely rescues the neuronal degeneration and significantly improves the light-elicited responses of the eye. The reduction need not be complete, suggesting that rescue of degeneration may be possible with minimal side effects arising from overdepletion of the target protein. (C) 2004 Elsevier B.V. All rights reserved.