Enhanced fluorescence of Cy5-labeled oligonucleotides near silver island films: A distance effect study using single molecule spectroscopy
Enhanced fluorescence of Cy5-labeled oligonucleotides near silver island films: A distance effect study using single molecule spectroscopy
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DOI：
10.1021/jp060402e
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发表时间：
2006-11-16
影响因子：
3.3
通讯作者：
Lakowicz, Joseph R.
中科院分区：
文献类型：
--
作者：
Fu, Yi;Lakowicz, Joseph R.
我们研究了荧光增强和寿命减少Cy 5探针分子在不同的距离从沉积的银岛膜表面使用单分子光谱方法。通过生物素化的牛血清白蛋白（BSA-生物素）和抗生物素蛋白的交替层，随后通过Cy 5标记的寡核苷酸结合到BSA-生物素层结构的顶部来控制荧光团分子与表面的接近度。我们观察到显着变化的亮度荧光团与距离金属结构，以及减少闪烁的存在下，银岛膜。此外，从单个分子的荧光寿命和表观发射强度的分布表明金属纳米结构附近的局部基质表面的不均匀性。这些研究说明了独家信息，否则隐藏在合奏测量。
We investigated fluorescence enhancements and lifetime reductions of Cy5 probe molecules at various distances from the deposited silver island film surface using single molecule spectroscopic methods. The proximity of fluorophore molecules to the surface was controlled by alternating layers of biotinylated bovine serum albumin (BSA-biotin) and avidin, followed by binding of Cy5-labeled oligonucleotides to the top of a BSA-biotin layer structure. We observed dramatically varied brightness of fluorophores with distances from metal structures as well with reduced blinking in the presence of silver island films. In addition, distributions of fluorescence lifetimes and apparent emission intensities from individual molecules indicate an inhomogeneous nature of local matrix surface near metallic nanostructures. These studies illustrate the exclusive information that is otherwise hidden in ensemble measurements.