EMILIN-1 regulates the amount of oxytalan fiber formation in periodontal ligaments in vitro
EMILIN-1 regulates the amount of oxytalan fiber formation in periodontal ligaments in vitro
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DOI：
10.3109/03008207.2010.502982
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发表时间：
2011-02-01
影响因子：
2.9
通讯作者：
Ishikawa, Hiroyuki
中科院分区：
文献类型：
--
作者：
Nakatomi, Yuka;Tsuruga, Eichi;Ishikawa, Hiroyuki
弹性系统纤维包括氧化他聚糖、伊春素和弹性纤维，它们的相对微纤维和弹性蛋白含量不同。其中，人牙周膜（PDL）只含有氧化纤维（纯微纤维）。弹性蛋白微原纤维界面定位蛋白-1（EMILIN-1）定位于微原纤维和弹性蛋白之间的界面。我们假设EMILIN-1可能有助于oxytalan纤维的形成。我们在PDL细胞培养物中使用针对EMILIN-1的小干扰RNA（siRNA）来检查EMILIN-1（微纤维的主要成分）的细胞外沉积。EMILIN-1被标记在对EMILIN-1阳性的微纤维上，并且在免疫沉淀测定时与EMILIN-1共定位。EMILIN-1抑制使EMILIN-1沉积水平降低至对照的23%，这是免疫荧光显示EMILIN-1沉积减少的原因。这些结果表明，EMILIN-1可能调节oxytalan纤维的形成，并在其稳态中发挥作用。
The elastic system fibers comprise oxytalan, elaunin, and elastic fibers, differing in their relative microfibril and elastin contents. Among them, human periodontal ligament (PDL) contains only oxytalan fibers (pure microfibrils). Elastin microfibril interface-located protein-1 (EMILIN-1) is localized at the interface between microfibrils and elastin. We hypothesized that EMILIN-1 may contribute to the formation of oxytalan fibers. We used a small interfering RNA (siRNA) for EMILIN-1 in PDL cell culture to examine the extracellular deposition of fibrillin-1(the major component of microfibrils). EMILIN-1 was labeled on microfibrils positive for fibrillin-1 and was colocalized with fibrillin-1 upon immunoprecipitation assay. EMILIN-1 suppression reduced the level of fibrillin-1 deposition to 23% of the control, and this was responsible for the diminution of fibrillin-1 deposition revealed by immunofluorescence. These results suggest that EMILIN-1 may regulate the formation of oxytalan fibers and play a role in their homeostasis.