Metabolome and Transcriptome Association Analysis Reveals Regulation of Flavonoid Biosynthesis by Overexpression of LaMIR166a in Larix kaempferi (Lamb.) Carr
Metabolome and Transcriptome Association Analysis Reveals Regulation of Flavonoid Biosynthesis by Overexpression of LaMIR166a in Larix kaempferi (Lamb.) Carr
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DOI：
10.3390/f11121367
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发表时间：
2020-12-01
期刊：
影响因子：
2.9
通讯作者：
Qi, Liwang
中科院分区：
文献类型：
--
作者：
Fan, Yanru;Li, Zhexin;Qi, Liwang
体细胞胚胎发生是研究植物早期发育的理想模式过程。日本落叶松胚胎细胞系的研究Carr过表达LaMIR166a在我们之前的研究中获得。本研究将从头转录组学和广泛靶向代谢组学相结合，用于研究野生型和lamir166a过表达的胚胎细胞系的转录谱和代谢变化。在野生型和转基因细胞系中共发现了459种代谢物。与野生型细胞系相比，lamir166a过表达细胞系的转录物和代谢物发生了显著变化。在差异表达基因（DEGs）中，苯丙氨酸和类黄酮合成基因显著富集，在差异积累代谢物（dam）中，酚酸和类黄酮积累量特别高。因此，类黄酮生物合成途径似乎是响应LaMIR166a过表达的最丰富的途径。基于京都基因与基因组百科数据库，对代谢组和转录组数据进行关联分析发现，mir166a过表达系的类黄酮生物合成和植物激素信号转导过程发生了显著变化，表明miR166可能参与了这些过程。本研究确定了一些与LaMIR166a过表达相关的潜在代谢物，为更好地理解miR166的调控机制提供了重要的基础。
Somatic embryogenesis is an ideal model process for studying early plant development. Embryonic cell lines of Larix kaempferi (Lamb.) Carr overexpressing LaMIR166a were obtained in our previous study. Here, a combination of de novo transcriptomics and extensively targeted metabolomics was used to study the transcriptional profiles and metabolic changes in wild-type and LaMIR166a-overexpressed embryonic cell lines. A total of 459 metabolites were found in the wild-type and transgenic cell lines. Compared to those in the wild-type cell lines, transcripts and metabolites were significantly altered in the LaMIR166a-overexpressed cell lines. Among differentially expressed genes (DEGs), phenylalanine and flavonoid synthesis genes were significantly enriched, and among differentially accumulated metabolites (DAMs), phenolic acids and flavonoids accumulated in particularly high amounts. Thus, the flavonoid biosynthetic pathway seems to be the most abundant pathway in response to LaMIR166a overexpression. Based on the Kyoto Encyclopedia of Genes and Genomes database, the association analysis of metabolome and transcriptome data showed that flavonoid biosynthesis and plant hormone signal transduction processes were significantly changed in miR166a-overexpression lines, suggesting that miR166 might be involved in these processes. The present study identified a number of potential metabolites associated with LaMIR166a overexpression, providing a significant foundation for a better understanding of the regulatory mechanisms underlying miR166.