In vitro effect of CTAB- and PEG-coated gold nanorods on the induction of eryptosis/erythroptosis in human erythrocytes
In vitro effect of CTAB- and PEG-coated gold nanorods on the induction of eryptosis/erythroptosis in human erythrocytes
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DOI：
10.3109/17435390.2011.625132
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发表时间：
2012-12-01
期刊：
影响因子：
5
通讯作者：
Kong, Siu K.
中科院分区：
文献类型：
--
作者：
Lau, Irene P.;Chen, Huanjun;Kong, Siu K.
金纳米棒（Au-NRs）由于其尺寸和独特的光学性质而引起了人们的极大兴趣。许多研究已经证明了它们在生物医学系统中的用途。然而，其潜在毒性尚未完全了解。本研究评价了人红细胞中用CTAB（十六烷基三甲基溴化铵）或PEG（聚乙二醇）包被的Au-NR（15 nM x 64 nM）对诱导溶血和红细胞凋亡的影响。在我们的研究中，通过流式细胞术测量细胞凋亡的特征事件，系统地确定红细胞凋亡（也称为eryptosis）。我们发现，CTAB和PEG包被的Au-NR高达0.5 nM不会引起严重的溶血。然而，CTAB-Au-NR比PEG-Au-NR毒性更大。CTAB-Au-NR的毒性主要是由于解吸或不完全纯化的CTAB残留物。从机制上讲，胞质Ca 2+离子似乎是由CTAB或CTAB-Au-NR介导的红细胞凋亡/红细胞凋亡中的关键介质，而caspase-3和活性氧对该过程没有多大贡献。
Gold nanorods (Au-NRs) have attracted enormous interest due to their size and unique optical properties. Many studies have demonstrated their use in biomedical systems. However, their potential toxicity is not fully understood. This study evaluated the effects of the Au-NRs (15 nM x 64 nM) coated with CTAB (cetyltrimethylammonium bromide) or PEG (polyethylene glycol) in human erythrocytes on the induction of haemolysis and erythroptosis. In our study, erythroptosis (also known as eryptosis) was determined systematically through the measurement of feature events of apoptosis by flow cytometry. We found that the CTAB-and PEG-coated Au-NRs up to 0.5 nM did not cause severe haemolysis. However, the CTAB-Au-NRs were more toxic than the PEG-Au-NRs. The toxicity of the CTAB-Au-NRs was largely due to the CTAB residues from desorption or incomplete purification. Mechanistically, cytosolic Ca2+ ions seem to be the key mediator in the eryptosis/erythroptosis mediated by the CTAB or CTAB-Au-NRs while caspase-3 and reactive oxygen species did not contribute much to the process.