Hypothalamic-pituitary vasculature: evidence for retrograde blood flow in the pituitary stalk.
Hypothalamic-pituitary vasculature: evidence for retrograde blood flow in the pituitary stalk.
复制标题
下丘脑-垂体脉管系统：垂体柄逆行血流的证据。
作者：
C. Oliver;R. S. Mical;J. C. Porter
在垂体完整的麻醉雄性大鼠和使用前30 - 60 min切除垂体前叶、垂体后叶或整个垂体的动物中，研究了垂体柄血管中垂体激素的逆行转运。通过微插管从单个长门静脉自由流动收集血液1.5至2小时，微插管的尖端指向下丘脑。在每次门静脉血采集结束时采集动脉血样。用放射免疫分析法测定垂体完整大鼠门静脉血浆中LH、TSH、催乳素、ACTH、α-MSH和加压素的浓度（ng/ml）如下：LH，2.320 ± 874（平均值和标准误）;促甲状腺激素，10，180 ± 1，471;催乳素，4，858 ± 884;促肾上腺皮质激素，82 ± 17.0; α-MSH，103 ± 17.8;加压素，2.4 ± 1.0。动脉血浆中LH <20，TSH 149 ± 22，催乳素25 ± 5.0，ACTH 0.36 ± 0.05，α-MSH 0.36 ± 0.05，α-MSH 0.05 ± 0.05，α-MSH 0.05 ± 0.05，α-MSH 0.05 ± 0.05，α-MSH 0.05 ± 0.05.
Retrograde transport of pituitary hormones in the pituitary stalk vasculature was investigated in anesthetized male rats in which the pituitary gland was intact and in animals in which the anterior pituitary, posterior pituitary, or entire pituitary gland had been removed 30 to 60 min before use. Blood was collected for 1.5 to 2 h by free flow from a single long portal vessel through a microcannula, the tip of which was pointed toward the hypothalamus. An arterial blood sample was obtained at the end of each collection of portal blood. The concentrations (ng\ml) of LH, TSH, prolactin, ACTH, α-MSH, and vasopressin, determined by radioimmunoassay, in portal plasma from rats with intact pituitary glands were as follows: LH, 2,320 ± 874 (mean and SE); TSH, 10,180 ± 1,471; prolactin, 4,858 ± 884; ACTH, 82 ± 17.0; a-MSH, 103 ± 17.8; vasopressin, 2.4 ± 1.0. The concentrations of these hormones in arterial plasma of these rats were as follows: LH, <20; TSH, 149 ± 22; prolactin, 25 ± 5.0; ACTH, 0.36 ± 0.05; α-MSH,...