CELLULAR-IMMUNITY IN EXPERIMENTAL ECHINOCOCCUS-MULTILOCULARIS INFECTION .1. SEQUENTIAL AND COMPARATIVE-STUDY OF SPECIFIC INVIVO DELAYED-TYPE HYPERSENSITIVITY AGAINST E-MULTILOCULARIS ANTIGENS IN RESISTANT AND SENSITIVE MICE
CELLULAR-IMMUNITY IN EXPERIMENTAL ECHINOCOCCUS-MULTILOCULARIS INFECTION .1. SEQUENTIAL AND COMPARATIVE-STUDY OF SPECIFIC INVIVO DELAYED-TYPE HYPERSENSITIVITY AGAINST E-MULTILOCULARIS ANTIGENS IN RESISTANT AND SENSITIVE MICE
复制标题
DOI：
10.1111/j.1365-2249.1990.tb05456.x
复制
发表时间：
1990-11-01
影响因子：
4.6
通讯作者：
VUITTON, DA
中科院分区：
文献类型：
--
作者：
LIANCE, M;BRESSONHADNI, S;VUITTON, DA
中间寄主对多房夜蛾幼虫接受性的种或品系差异可能与寄主特异性细胞免疫应答的差异有关。为了验证这一假设，我们评估了三种不同敏感性的小鼠（AKR和C57BL）对多房绦虫抗原（EmAg）的延迟型超敏反应（DTH）。6)或对多房大肠杆菌感染的耐药性（C57BL.10）。在EmAg抗原刺激24小时后，通过测量足垫在体内的反应来测定DTH。评价免疫小鼠的阳性反应水平；然而，典型的DTH反应仅在使用强佐剂计划免疫小鼠时观察到。免疫小鼠甲状旁腺激素的病程在敏感小鼠和抗性小鼠中有所不同。在感染增殖性多房棘球绦虫幼虫的小鼠中，这种差异更为明显。耐药小鼠的足垫反应水平明显高于敏感小鼠，但反应高峰出现的时间晚于敏感小鼠。通过足垫对EmAg的反应表达的DTH在敏感和耐药小鼠的整个观察期间都保持显著。小鼠宿主的接受性与抗EmAg的特异性抗体水平之间没有相关性。这些结果提示小鼠对多房芽胞杆菌感染的抗性与DTH的强度和/或病程之间存在关系。这种抗性可能是由周围寄生肉芽肿的一些特异性的原位细胞免疫反应介导的。
Species- or strain-related differences in receptivity of intermediate hosts to E. multilocularis larvae could be related to differences in specific cellular immune response of the host. In order to test this hypothesis, we assessed the delayed-type hypersensitivity (DTH) to E. multilocularis antigens (EmAg) in mice of three strains differing by their sensitivity (AKR and C57BL.6) or resistance (C57BL.10) to E. multilocularis infection. DTH was determined by measuring in vivo the foot-pad response 24 h after an EmAg antigenic challenge. The level of positive response was evaluated in immunized mice; however, a typical DTH response was only observed by immunizing mice with a strong adjuvant schedule. Course of DTH in the immunized mice was shown to be somewhat different in sensitive and resistant mice. The differences were much more marked in mice infected with proliferative E. multilocularis larvae. The levels of the footpad response was significantly higher in resistant mice, although the peak of the reaction was obtained later than in sensitive mice. DTH, expressed by the foot-pad response against EmAg, remained significant for the entire period of observation in sensitive as well as in resistant mice. There was no correlation between receptivity of the murine hosts and levels of specific antibodies against EmAg. These results suggest a relationship between resistance to E. multilocularis infection and intensity and/or course of DTH in mice. The resistance could be mediated by some particularities of the in situ cellular immune response in the periparasitic granuloma.