Caveolae-mediated endocytosis of conjugated polymer nanoparticles.
Caveolae-mediated endocytosis of conjugated polymer nanoparticles.
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DOI：
10.1002/mabi.201300030
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发表时间：
2013-07
影响因子：
4.6
通讯作者：
Moon JH
中科院分区：
文献类型：
--
作者：
Lee J;Twomey M;Machado C;Gomez G;Doshi M;Gesquiere AJ;Moon JH
了解合成生物材料的细胞相互作用和进入途径对于提高整体标记和递送效率非常重要。共轭聚合物纳米颗粒（CPN）是新兴的荧光材料，其已用于癌细胞标记和小干扰RNA（siRNA）递送。在这方面的贡献，详细的生物物理性质的CPNs，包括进入机制和亚细胞定位进行了研究，使用荧光为基础的技术。虽然CPN不引起毒性，但从用染料木黄酮预处理的癌细胞中观察到CPN摄取减少，染料木黄酮是小窝介导的内吞作用（CvME）的抑制剂。CvME通过与小窝蛋白-1蛋白的高度共定位进一步证实，所述小窝蛋白-1蛋白存在于小窝和小窝体中。优异的生物物理性质、无毒性和非破坏性递送途径支持CPN是有前途的多功能载体，其最小化递送期间内容物的降解。
Understanding cellular interactions and entry pathways of synthetic biomaterials are highly important to improve overall labeling and delivery efficiency. Conjugated polymer nanoparticles (CPNs) are emerging, fluorescent materials that have been used for cancer cell labeling and small interfering RNA (siRNA) delivery. In this contribution, detailed biophysical properties of CPNs including entry mechanisms and subcellular localization were studied using fluorescent-based techniques. While CPNs cause no toxicity, decreased CPN uptake was observed from cancer cells pretreated with genistein, which is an inhibitor of caveolae-mediated endocytosis (CvME). CvME was further confirmed by high co-localization with caveolin-1 proteins, which are found in the caveolae and caveosomes. Excellent photophysical properties, non-toxicity, and non-destructive delivery pathways support that CPNs are promising multifunctional carriers minimizing degradation of contents during delivery.
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影响因子：
16.6
作者：
Moon, Joong Ho;McDaniel, William;Hancock, Lawrence E.
通讯作者：
Hancock, Lawrence E.
影响因子：
15
作者：
Srinivasachari, Sathya;Fichter, Katye M.;Reineke, Theresa M.
通讯作者：
Reineke, Theresa M.
影响因子：
8.6
作者：
Tan, Shawn J.;Jana, Nikhil R.;Ying, Jackie Y.
通讯作者：
Ying, Jackie Y.
影响因子：
5.3
作者：
Silva AT;Nguyen A;Ye C;Verchot J;Moon JH
通讯作者：
Moon JH
影响因子：
10.8
作者：
Choi, JS;Nam, K;Park, J
通讯作者：
Park, J