ANTIGENIC STRUCTURE OF POLIOVIRUSES OF SEROTYPE-1, SEROTYPE-2 AND SEROTYPE-3
ANTIGENIC STRUCTURE OF POLIOVIRUSES OF SEROTYPE-1, SEROTYPE-2 AND SEROTYPE-3
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DOI：
10.1099/0022-1317-67-7-1283
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发表时间：
1986-07-01
影响因子：
3.8
通讯作者：
ICENOGLE, JP
中科院分区：
文献类型：
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作者：
MINOR, PD;FERGUSON, M;ICENOGLE, JP
通过分离和鉴定抗中和突变体，研究了1、2和3血清型脊髓灰质炎病毒Sabin疫苗株具有中和活性的单克隆抗体识别的抗原位点。已经确定了三个不同的地点，分别为地点1、地点2和地点3。位点1包括VPI的12个氨基酸的区域，从残基89到100，VPI的相应区域已被确定为脊髓灰质炎病毒2的抗原位点。该位点在2型和3型脊髓灰质炎病毒中具有很强的免疫优势，但在1型脊髓灰质炎病毒中未检测到。位点2是一个复杂的位点，包括VP1的残基220到222（位点2a），残基包括VP2的169和170等（位点2b）。位点2a和位点2b已在1型脊髓灰质炎病毒中检测到，而位点2b仅在3型脊髓灰质炎病毒中检测到。位点3是一个复合位点，包括VP1的残基286至290（位点3a），残基包括VP3的58、59和其他残基（位点3b）。已在3型脊髓灰质炎病毒中检测到3a和3b位点，而在1型脊髓灰质炎病毒中仅检测到3b位点。
The antigenic sites recognized by monoclonal antibodies with neutralizing activity for the Sabin vaccine strains of poliovirus of serotypes 1,2 and 3 have been studied by the isolation and characterization of mutants resistant to neutralization by antibody. Three distinct sites have been identified which are designated site 1, site 2 and site 3. Site 1 includes a region of 12 amino acids of VPI, from residues 89 to 100, and a corresponding region of VPI has been identified as an antigenic site for poliovirus 2. This site was strongly immunodominant in type 2 and type 3 but was not detected for poliovirus 1. Site 2 is a complex site including residues 220 to 222 from VP1 (site 2a) with residues including 169 and 170 and others of VP2 (site 2b). Both site 2a and site 2b have been detected in type 1 poliovirus, while as yet only site 2b has been detected in type 3 poliovirus. Site 3 is a complex site including residues 286 to 290 from VP1 (site 3a) with residues including 58 and 59 and others of VP3 (site 3b). Both sites 3a and 3b have been detected in type 3 poliovirus, while as yet only site 3b has been detected in type 1 poliovirus.