B-box transcription factor 28 regulates flowering by interacting with constans.
B-box transcription factor 28 regulates flowering by interacting with constans.
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B-box 转录因子 28 通过与常量相互作用调节开花
DOI：
10.1038/s41598-020-74445-7
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发表时间：
2020-10-20
影响因子：
4.6
通讯作者：
Fang Y
中科院分区：
文献类型：
--
作者：
Liu Y;Lin G;Yin C;Fang Y
B-box转录因子（BBXs）是植物开花、光形态发生、避荫、非生物和生物胁迫以及激素通路的重要调控因子。在拟南芥中，已经鉴定出32种BBX蛋白，并根据其结构域将其分为5类。目前对第五族成员BBX26-BBX32及其功能相关的详细分子机制知之甚少。在这里，我们发现B-box转录因子28 （BBX28）与Constans （CO）相互作用，Constans是开花位点T （FT）的转录激活因子。过表达BBX28导致开花晚，FT转录显著降低，BBX28缺陷突变体在长日（long days， LD）条件下表现出较弱的早花表型，说明BBX28在长日（long days， LD）条件下在开花过程中发挥了负性和冗余性作用。此外，BBX28与CO的相互作用减少了CO向FT位点的募集，但不影响CO的转录激活活性。特别是B-box结构域内的那些，对于BBX28和CO之间的异源二聚化以及CO在FT转录上的激活是必不可少的。遗传证据表明，BBX28过表达引起的延迟开花受到CO异位表达的损害。综上所述，这些结果支持BBX28与CO和FT一起负调控拟南芥开花，其中BBX28的n端保守半胱氨酸可能起核心作用。
B-box transcription factors (BBXs) are important regulators of flowering, photomorphogenesis, shade-avoidance, abiotic and biotic stresses and plant hormonal pathways. In Arabidopsis, 32 BBX proteins have been identified and classified into five groups based on their structural domains. Little is known about the fifth group members (BBX26–BBX32) and the detailed molecular mechanisms relevant to their functions. Here we identified B-box transcription factor 28 (BBX28) that interacts with Constans (CO), a transcriptional activator of Flowering Locus T (FT). Overexpressing BBX28 leads to late flowering with dramatically decreased FT transcription, and bbx28 deficient mutant displays a weak early flowering phenotype under long days (LD), indicating that BBX28 plays a negative and redundant role in flowering under LD. Additionally, the interaction between BBX28 and CO decreases the recruitment of CO to FT locus without affecting the transcriptional activation activity of CO. Moreover, the N-terminal cysteines, especially those within the B-box domain, are indispensable for the heterodimerization between BBX28 and CO and activation of CO on FT transcription. Genetic evidences show that the later flowering caused by BBX28 overexpression is compromised by CO ectopic expression. Collectively, these results supported that BBX28 functions with CO and FT to negatively regulate Arabidopsis flowering, in which the N-terminal conserved cysteines of BBX28 might play a central role.
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