First use of oceanic environmental DNA to study the spawning ecology of the Japanese eel Anguilla japonica
First use of oceanic environmental DNA to study the spawning ecology of the Japanese eel Anguilla japonica
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DOI：
10.3354/meps12828
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发表时间：
2019-01-17
影响因子：
2.5
通讯作者：
Tsukamoto, Katsumi
中科院分区：
文献类型：
--
作者：
Takeuchi, Aya;Watanabe, Shun;Tsukamoto, Katsumi
环境DNA（eDNA）分析正在成为一种创新的工具，以评估物种在水生环境中的分布和生物量，使快速和非侵入性的调查相比，传统的采样方法，如捕鱼，水声，潜水观测。首次将eDNA分析应用于北太平洋西部日本鳗鲡产卵生态学研究。初步的实验室实验，使用水箱水含有鳗鱼证实，我们的特异性引物和探针可以识别日本鳗鱼从少量的eDNA（约0.5 ng/PCR反应）。在2015年5月19日至20日在西马里亚纳海脊南部进行的海洋调查期间，在A产卵区的9个站点的12个水深处收集了海水样本。日本。在108个海水样品中，从250和400 m处采集的3个样品中成功地扩增了DNA。根据已发表的关于日本鳗鱼产卵生态学和eDNA动态的研究，从海洋调查中获得的eDNA阳性信号可能来自成年日本鳗鱼或来自最近一次产卵事件的幼鳗。因此，船上的eDNA分析被证明是一个可行的方法来检测日本鳗鱼在其产卵区沿着南部西马里亚纳海脊。
Environmental DNA (eDNA) analysis is emerging as an innovative tool to assess the distribution and biomass of species in aquatic environments that enables quick and non-invasive surveys compared to conventional sampling methods such as fishing, hydroacoustics, and diving observations. We applied eDNA analysis to spawning ecology research of the Japanese eel Anguilla japonica in the western North Pacific for the first time. A preliminary laboratory experiment using tank water containing eels confirmed that our specific primers and probe could identify the Japanese eel from a small amount of eDNA (about 0.5 ng per PCR reaction). During a 19-20 May 2015 ocean survey at the southern West Mariana Ridge, seawater samples were collected at 12 water depths at 9 stations in the spawning area of A. japonica. Out of 108 seawater samples, DNA was successfully amplified from 3 samples, which were collected at 250 and 400 m. According to published research on Japanese eel spawning ecology and eDNA dynamics, the eDNA positive signals obtained from the oceanic survey could have been from adult Japanese eels or from larvae from the last spawning events. Thus onboard eDNA analysis proved to be a viable approach for detecting Japanese eels in their spawning area along the southern West Mariana Ridge.