High expression of complement components in omental adipose tissue in obese men
High expression of complement components in omental adipose tissue in obese men
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DOI：
10.1038/oby.2003.100
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发表时间：
2003-06-01
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影响因子：
--
通讯作者：
Carlsson, LMS
中科院分区：
文献类型：
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作者：
Gabrielsson, BG;Johansson, JM;Carlsson, LMS
目的：内脏脂肪蓄积被认为是肥胖相关代谢紊乱的预测因子。主要在这个仓库表达的因素可以介导内脏肥胖和相关疾病之间的联系。研究方法和程序：配对的皮下和网膜脂肪组织活检，从10个肥胖的男性。通过DNA微阵列一式三份和实时聚合酶链反应分析基因表达。采用放射免疫扩散法分析了91例代表一般人群横断面的受试者的血清C3和C4。结果：补体成分C2、C3、C4、C7和因子B在网膜中的表达量分别是皮下脂肪的2、4、17、10和7倍。此外，adipsin，这属于替代途径，和经典途径组件C1 QB，C1 R，和C1 S在两个仓库中表达。组织分布分析显示，C2、C3和C4在网膜脂肪组织中高表达，并且只有肝脏具有这些基因的较高表达。两名男性的血清C3水平均与内脏和皮下脂肪组织相关（分别为r = 0.65和p < 0.001和r = 0.52和p < 0.001）和女性（分别为r = 0.34和p = 0.023和r = 0.49和p < 0.001），而男性中C4水平仅与内脏脂肪相关（r = 0.36，p = 0.015）和在女性中的两种贮库（内脏：r = 0.58，p < 0.001;和皮下：r = 0.51，p < 0.001）。腹腔内脂肪组织中补体基因的高表达可能表明补体系统参与内脏脂肪症的发展和/或有助于与内脏脂肪量增加相关的代谢并发症。
Objective: Accumulation of visceral fat is recognized as a predictor of obesity-related metabolic disturbances. Factors that are predominantly expressed in this depot could mediate the link between visceral obesity and associated diseases.Research Methods and Procedures: Paired subcutaneous and omental adipose tissue biopsies were obtained from 10 obese men. Gene expression was analyzed by DNA microarrays in triplicate and by real-time polymerase chain reaction. Serum C3 and C4 were analyzed by radial immunodiffusion assays in 91 subjects representing a cross section of the general population. Body composition was measured by computerized tomography.Results: Complement components C2, C3, C4, C7, and Factor B had higher expression in omental compared with subcutaneous adipose tissue (similar to2-, 4-, 17-, 10-, and 7-fold, respectively). In addition, adipsin, which belongs to the alternative pathway, and the classical pathway components C1QB, C1R, and C1S were expressed in both depots. Analysis of tissue distribution showed high expression of C2, C3, and C4 in omental adipose tissue, and only liver had higher expression of these genes. Serum C3 levels correlated with both visceral and subcutaneous adipose tissue in both men (r = 0.65 and p < 0.001 and r = 0.52 and p < 0.001, respectively) and women (r = 0.34 and p = 0.023 and r = 0.49 and p < 0.001, respectively), whereas C4 levels correlated with only visceral fat in men (r = 0.36, p = 0.015) and with both depots in women (visceral: r = 0.58, p < 0.001; and subcutaneous: r = 0.51, p < 0.001).Discussion: Recent studies show that the metabolic syndrome is associated with chronically elevated levels of several immune markers, some of which may have metabolic effects. The high expression of complement genes in intra-abdominal adipose tissue might suggest that the complement system is involved in the development of visceral adiposity and/or contributes to the metabolic complications associated with increased visceral fat mass.