Expression of lactate dehydrogenase C in MDA-MB-231 cells and its role in tumor invasion and migration
Expression of lactate dehydrogenase C in MDA-MB-231 cells and its role in tumor invasion and migration
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DOI：
10.3892/mmr.2016.4963
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发表时间：
2016-04-01
影响因子：
3.4
通讯作者：
Lin, Donghong
中科院分区：
文献类型：
--
作者：
Kong, Lingying;Du, Wei;Lin, Donghong
癌/睾丸抗原（CTA）乳酸脱氢酶C（LDHC）是哺乳动物生殖细胞中与葡萄糖和三磷酸腺苷产生相关的独特LDH同工酶。然而，LDHC在癌症中的作用迄今为止在很大程度上仍然难以捉摸。本研究探讨LDHC在人乳腺癌MDA-MB-231细胞中的表达状况及其在肿瘤侵袭和迁移中的作用。免疫组织化学分析显示，内源性LDHC表达在MDA-MB-231细胞的细胞质和细胞核中产生。此外，体外细胞侵袭和迁移试验表明，当LDHC表达被其特异性抑制剂阻断时，MDA-MB-231细胞的细胞侵袭和迁移受到损害。值得注意的是，LDHC的抑制不能诱导MDA-MB-231细胞的凋亡。本研究提供的证据表明，LDHC酶作为CTA在乳腺癌中发挥作用，并在肿瘤的侵袭和迁移发挥重要作用。
The cancer/testis antigen (CTA) lactate dehydrogenase C (LDHC) is a unique LDH isoenzyme associated with glucose and adenosine triphosphate production in mammalian germ cells. However, the role of LDHC in cancer has thus far largely remained elusive. The present study described the expression status of LDHC in human MDA-MB-231 breast cancer cells as well as its role in tumor invasion and migration. Immunohistochemical analysis revealed endogenous LDHC expression in the cytoplasm and nuclei of MDA-MB-231 cells yielded. In addition, in vitro cell invasion and migration assays revealed that when LDHC expression was blocked by its specific inhibitor, cell invasion and migration were compromised in MDA-MB-231 cells. Of note, inhibition of LDHC was unable to induce apoptosis in MDA-MB-231 cells. The present study provided evidence that the LDHC enzyme acts as a CTA in breast carcinoma and exerts an essential role in tumor invasion and migration.