Polarization behavior of bone marrow-derived macrophages on charged P(VDF-TrFE) coatings.
Polarization behavior of bone marrow-derived macrophages on charged P(VDF-TrFE) coatings.
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DOI：
10.1039/d0bm01604g
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发表时间：
2020-11
影响因子：
6.6
通讯作者：
Zhiying Wang;Xuzhao He;Bolin Tang;Xiaoyi Chen;Lingqing Dong;Kui Cheng;W. Weng
中科院分区：
文献类型：
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作者：
Zhiying Wang;Xuzhao He;Bolin Tang;Xiaoyi Chen;Lingqing Dong;Kui Cheng;W. Weng
骨植入物的免疫反应与巨噬细胞与生物材料表面的相互作用密切相关。本文系统研究了小鼠骨髓源性巨噬细胞（BMDMs）在不同电位强度的带电表面上的极化行为，包括其形态、表型标志物、基因和细胞因子的表达。我们发现，荷电表面可以有效地促进BMDM极化，更高的电位强度有利于上调BMDM的极化为M2表型。通过对整合素（αMβ2和α5β1）和钾离子通道（Kv1.3）信号通路的分析，发现整合素来源的信号通路更能主导巨噬细胞向M2表型的极化。因此，本工作表明，表面电荷，作为一种物理化学性质的材料表面，可以有效地调节巨噬细胞极化，这可能提供了一个免疫调节的角度为生物材料的表面设计。
The immune response of bone implants is closely related to the interaction between macrophages and biomaterial surfaces. In this work, the polarization behavior of mouse bone marrow-derived macrophages (BMDMs), including their morphology and expression of phenotypic markers, genes and cytokines, on charged surfaces with different potential intensities was systematically explored. We found that the charged surface could effectively promote BMDM polarization, and a higher potential intensity was conducive to the upregulation of the polarization of BMDMs into the M2 phenotype. Based on the analysis of the signaling pathways involved in integrins (αMβ2 and α5β1) and the potassium ion channel (Kv1.3), a possible underlying mechanism revealed that the integrin originated signaling pathways could more dominantly regulate macrophage polarization to the M2 phenotype. The present work therefore demonstrates that the surface charge, as a physicochemical property of material surfaces, could effectively regulate macrophage polarizations, which may provide an immunoregulation view for the surface design of biomaterials.