Cyanine fluorochrome-labeled antibodies in vivo: Assessment of tumor imaging using Cy3, Cy5, Cy5.5, and Cy7
Cyanine fluorochrome-labeled antibodies in vivo: Assessment of tumor imaging using Cy3, Cy5, Cy5.5, and Cy7
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DOI：
10.1046/j.1525-1500.1998.0oa25.x
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发表时间：
1998-01-01
影响因子：
--
通讯作者：
Farkas, DL
中科院分区：
文献类型：
--
作者：
Ballou, B;Fisher, GW;Farkas, DL
针对两种不同可靶向抗原的单克隆抗体与四种市售的菁荧光染料中的每一种进行偶联。将等量的所有四种抗体共同注射到荷瘤动物体内并进行成像。所有四种荧光染料都能对小型浅表肿瘤进行充分标记。大型肿瘤仅能被Cy7很好地标记，这可能是由于自身遮蔽和稀释效应。对于观察动物体内深处的结构，Cy7优于其他菁荧光染料。
Monoclonal antibodies to two different targetable antigens were conjugated to each of four commercially available cyanine fluorochromes. Equal amounts of all four antibodies were coinjected into tumor-bearing animals and imaged. Small, superficial tumors were adequately labeled using all four fluorochromes. Large tumors were labeled well only by Cy7, probably due to self-masking and dilution effects. Cy7 was superior to other cyanine fluorochromes for visualizing structures located deep within the animal.