The Arabidopsis CBF gene family is composed of three genes encoding AP2 domain-containing proteins whose expression is regulated by low temperature but not by abscisic acid or dehydration
The Arabidopsis CBF gene family is composed of three genes encoding AP2 domain-containing proteins whose expression is regulated by low temperature but not by abscisic acid or dehydration
复制标题
DOI：
10.1104/pp.119.2.463
复制
发表时间：
1999-02-01
期刊：
影响因子：
7.4
通讯作者：
Salinas, J
中科院分区：
文献类型：
--
作者：
Medina, J;Bargues, M;Salinas, J
我们已经从拟南芥中发现了两个与CBF1高度相似的基因，CBF1是一个编码AP2结构域转录激活因子的基因，该基因与低温响应元件CCGAC结合，诱导一些冷调控基因的表达，提高植物的Greeting耐受性。我们将这两个基因命名为CBF2和CBF3，它们也编码含有AP2 dna结合基序的蛋白质。此外，与CBF1一样，CBF2和CBF3蛋白也包含假定的核定位信号和潜在的酸性激活域。CBF2和CBF3基因与CBF1相连，构成了4号染色体下臂上的一个簇。三个CBF基因之间的高度相似性、它们的串联组织以及它们具有相同的转录取向都表明它们有共同的起源。CBF1、CBF2和CBF3表现出相同的表达模式，在低温处理下被快速诱导。然而，与大多数已知的冷诱导植物基因不同，它们对脱落酸或脱水没有反应。综上所述，所有这些数据表明CBF2和CBF3可能作为转录激活因子，通过脱落酸不依赖的途径控制低温基因表达水平并促进抗冻能力。
We have identified two genes from Arabidopsis that show high similarity with CBF1, a gene encoding an AP2 domain-containing transcriptional activator that binds to the low-temperature-responsive element CCGAC and induces the expression of some cold-regulated genes, increasing plant Greeting tolerance. These two genes, which we have named CBF2 and CBF3, also encode proteins containing AP2 DNA-binding motifs. Furthermore, like CBF1, CBF2 and CBF3 proteins also include putative nuclear-localization signals and potential acidic activation domains. The CBF2 and CBF3 genes are linked to CBF1, constituting a cluster on the bottom arm of chromosome IV. The high level of similarity among the three CBF genes, their tandem organization, and the fact that they have the same transcriptional orientation all suggest a common origin. CBF1, CBF2, and CBF3 show identical expression patterns, being induced very rapidly by low-temperature treatment. However, in contrast to most of the cold-induced plant genes characterized, they are not responsive to abscisic acid or dehydration. Taken together, all of these data suggest that CBF2 and CBF3 may function as transcriptional activators, controlling the level of low-temperature gene expression and promoting freezing tolerance through an abscisic acid-independent pathway.