VESTIBULAR PRIMARY AFFERENT PROJECTION TO THE CEREBELLUM OF THE RABBIT
VESTIBULAR PRIMARY AFFERENT PROJECTION TO THE CEREBELLUM OF THE RABBIT
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DOI：
10.1002/cne.903270405
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发表时间：
1993-01-22
影响因子：
2.5
通讯作者：
SHOJAKU, H
中科院分区：
文献类型：
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作者：
BARMACK, NH;BAUGHMAN, RW;SHOJAKU, H
用逆行和顺行示踪法研究了家兔前庭初级传入纤维向小脑的投射。我们用辣根过氧化物酶（HRP）或麦胚凝集素-HRP（WGA-HRP）注射小脑小叶。在这些注射后，对斯卡帕神经节中标记和未标记细胞的数量进行计数。在悬雍垂结节注射后，约64-89%的斯卡帕神经节细胞被逆行标记。注射小叶后，同侧Scarpa神经节中约2%的细胞被标记。几乎没有细胞被标记的腹侧parafloculus.The前庭初级传入投射悬雍垂结节是如此广泛，它必须是一个侧支系统，也支配前庭核的一部分。通过将荧光示踪剂注射到悬雍垂结节和前庭复合体中证实了这种侧支投射模式。荧光金注入悬雍垂结节和过氧化物酶-罗丹明异硫氰酸盐注入前庭复合体。用破伤风毒素C片段（TTC）作顺行示踪剂，证实了前庭初级传入纤维向悬雍垂-小结的密集投射。用TTC技术，前庭初级传入投射到悬雍垂结节只终止于同侧颗粒细胞层的小叶9d和10。前庭初级传入纤维投射在小脑大沟内较稀疏。一个几乎检测不到的投影被发现的绒球和腹侧副绒球。
The vestibular primary afferent projection to the cerebellum of the rabbit was studied with retrograde and orthograde tracers. We injected individual lobules of the cerebellum with horseradish peroxidase (HRP) or wheat germ agglutinin-HRP (WGA-HRP). Following these injections the numbers of labeled and unlabeled cells in Scarpa's ganglion were counted. Approximately 64-89% of the cells in Scarpa's ganglion were labeled retrogradely following uvula-nodular injections. About 2% of the cells in the ipsilateral Scarpa's ganglion were labeled after injections of the flocculus. Virtually no cells were labeled following injections of the ventral paraflocculus.The vestibular primary afferent projection to the uvula-nodulus is so extensive that it must be part of a collateral system that also innervates the vestibular nuclei. This collateral projection pattern was confirmed by using fluorescent tracers injected into the uvula-nodulus and vestibular complex. Fluorogold was injected into the uvula-nodulus and peroxidase-rhodamine isothiocyanate was injected into the vestibular complex. More than 50% of the neurons in Scarpa's ganglion were double labeled by these subtotal injections.The dense vestibular primary afferent projection to the uvula-nodulus was confirmed by using the C fragment of tetanus toxin (TTC) injected into the labyrinth as an orthograde tracer. With the TTC technique, the vestibular primary afferent projection to the uvula-nodulus terminated exclusively in the ipsilateral granule cell layer of lobules 9d and 10. Much sparser vestibular primary afferent projections were found in the banks of major cerebellar sulci. A barely detectable projection was found to the flocculus and ventral paraflocculus.