Heterogeneity of ribosomal proteins among Streptomyces species and its application to identification.
Heterogeneity of ribosomal proteins among Streptomyces species and its application to identification.
复制标题
链霉菌属核糖体蛋白的异质性及其鉴定应用。
DOI：
10.1099/00221287-135-10-2635
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
K. Ochi
中科院分区：
文献类型：
--
作者：
K. Ochi
用双向PAGE对11株链霉菌的核糖体蛋白进行了比较。蛋白质图谱对每个物种都是特定的，不受吖啶染料处理的影响，这表明核糖体蛋白质的遗传稳定性。通过传统的分类方法和核糖体蛋白图谱分析，对一株链霉菌进行了鉴定。两种方法都将该菌株鉴定为淡黄色链霉菌，蛋白质图谱分析也表明该菌株与该物种有密切的亲缘关系。从而论证了核糖体蛋白图谱在链霉菌分类中的实际应用。
The ribosomal proteins from 11 Streptomyces strains representing various numerical taxonomic clusters were compared by two-dimensional PAGE. The protein patterns were specific for each species and were unaffected by acridine dye treatment, suggesting genetic stability of ribosomal proteins. An attempt was made to identify one strain of Streptomyces by both traditional taxonomic methods and analysis of the ribosomal protein patterns. Both methods identified the strain as Streptomyces lavendulae, and protein pattern analysis also showed that Streptomyces avidinii was closely related to this species. The practical application of ribosomal protein patterns in Streptomyces taxonomy was therefore demonstrated.