Effects of two antihypertensive agents, labetalol and metoprolol, on the production of reactive oxygen species by normal polymorphonuclear leukocytes in vitro.
Effects of two antihypertensive agents, labetalol and metoprolol, on the production of reactive oxygen species by normal polymorphonuclear leukocytes in vitro.
复制标题
两种抗高血压药物拉贝洛尔和美托洛尔对体外正常多形核白细胞产生活性氧的影响。
作者：
R. Jaboureck;B. Gressier;T. Dine;C. Brunet;M. Luyckx;P. Harfaut;L. Ballester;M. Cazin;J. Cazin
客观的
OBJECTIVE
Increased lipid peroxidation is a putative causal factor for preeclampsia. Because oxygen free radicals are involved in inducing the lipid oxidation chain reaction, we evaluated two beta adrenoreceptor blockers, labetalol and metoprolol, currently used for treating hypertension with regard to their ability to inhibit the formation of reactive oxygen species during respiratory burst of human normal polymorphonuclear leukocytes in vitro.
METHODS
We determined whether labetalol or metoprolol have antioxidative activity in a model of polymorphonuclear leukocytes stimulated in vitro with phorbol-12-myristate-13-acetate (PMA) and N-formyl-methionin-leucin-phenylalanin (fMLP). We also studied the scavenging properties of these two drugs using acellular systems.
RESULTS
Labetalol inhibits O2-. production by neutrophils activated by fMLP (IC50 = 17.5 mg/L) and weakly by PMA (43.6% inhibition at 100 mg/L). It also possesses a significant activity on OH. production (IC50 = 65 mg/L) that may depend in part on its ability to interfere with iron in the Fenton reaction. The same assays performed with metoprolol did not show any inhibitory effect on O2.- generation. It decreased weak OH. production by neutrophils, as a result of cellular and scavenging effects.
CONCLUSION
Labetalol shows important antioxidative properties in vitro with regard to normal leukocyte oxidative metabolism.
蛋白激酶 C 和 NADPH 氧化酶复合物成分之间的相互作用可能会调节佛波酯刺激的中性粒细胞产生的超氧化物。
DOI：
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发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Curnutte,JT;Erickson,RW;Ding,J;Badwey,JA
通讯作者：
Badwey,JA
人中性粒细胞中磷脂酶 A2 的激活。
DOI：
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发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Bauldry,SA;Wykle,RL;Bass,DA
通讯作者：
Bass,DA