Identification and distribution of actin in spermatogenic cells and spermatozoa of the rabbit.
Identification and distribution of actin in spermatogenic cells and spermatozoa of the rabbit.
复制标题
兔生精细胞和精子中肌动蛋白的鉴定和分布。
DOI：
10.1016/0012-1606(85)90467-1
复制
发表时间：
1985
影响因子：
2.7
通讯作者：
O'Rand,MG
中科院分区：
文献类型：
--
作者：
Welch,JE;O'Rand,MG
利用单克隆抗体作为高度特异性探针和兔射精精子的无精粒部分，肌动蛋白被定位于成熟兔精子顶体后区域。精子肌动蛋白已被提取出来，并在二维PAGE免疫印迹上鉴定为pI = 5.45, mr = 43,000的单个点。兔精子肌动蛋白以非丝状形式存在，不能通过去除质膜来去除。然而，与成熟精子不同的是，丝状肌动蛋白存在于生精细胞中，这是通过罗丹明阴茎素染色确定的。肌动蛋白开始在精母细胞中弥漫性分布，在顶体下空间积累，并与发育中的顶体一起呈带状。这条带在整个精子期延长，并在睾丸精子顶体后区域染色时变得连续。因此，肌动蛋白可能在精子发生过程中发挥作用，使顶体形成细胞核，并锚定顶体内膜蛋白。
Using a monoclonal antibody as a highly specific probe and a seminal particle-free fraction of rabbit ejaculated spermatozoa, actin has been localized in the postacrosomal region of mature rabbit spermatozoa. The sperm actin has been extracted and identified on two-dimensional PAGE immunoblots as a single spot of pI = 5.45 andMr= 43,000. Rabbit sperm actin is present in a nonfilamentous form and is not removed by removing the plasma membrane. Unlike mature spermatozoa, however, filamentous actin is present in spermatogenic cells, as determined by rhodamine phalloidin staining. Starting as diffusely distributed in spermatocytes, actin accumulates in the subacrosomal space and appears as a band in conjunction with the developing acrosome. This band lengthens throughout the spermatid stage and becomes continuous with the postacrosomal region staining in testicular spermatozoa. Actin may therefore function during spermatogenesis to both shape the acrosome to the nucleus and to anchor inner acrosomal membrane proteins.