Miniaturized gel electrophoresis system for fast separation of nucleic acids
Miniaturized gel electrophoresis system for fast separation of nucleic acids
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DOI：
10.1016/j.snb.2017.07.064
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发表时间：
2018-01-01
影响因子：
8.4
通讯作者：
Yamaguchi, Yoshinori
中科院分区：
文献类型：
--
作者：
Li, Zhenqing;Huang, Jiaxin;Yamaguchi, Yoshinori
平板凝胶电泳（SGE）是DNA、RNA和蛋白质分析的常用工具，但它繁琐、劳动密集、依赖技能且相对缓慢。为此，我们研制了一种基于生物芯片的SGE系统，该系统可以解析DNA片段并记录其分离过程。通过50 bp的DNA梯状电泳，我们发现16个DNA片段在14 min内就被有效地解析。例如牙龈卟啉单胞菌（P.g）、Tannerela gingivalis（T.f）和齿垢密螺旋体la（T.d））通过分离它们的聚合酶链反应产物。实验表明，该系统可在12 min内对P.g.、T.d.和T.f.进行诊断，P.g.的电泳检测结果表明，该系统的检测限约为6.4 ng/μ l。该系统成本低，操作方便，能有效地提高生物实验中的SGE，尤其适用于第三世界国家的实验室。(C)2017由Elsevier B. V.出版
Slab gel electrophoresis (SGE) is very common tool for DNA, RNA and protein analysis, but it is tedious, labor-intensive, skill-dependent, and relatively slow. Herein, we developed a biochip based SGE system, which can resolve the DNA fragments and record their separation process. By electrophoresis of 50 bp DNA ladder, we found that the 16 DNA fragments were effectively resolved within 14 min. In order to validate its feasibility and practicability, we take periodontal pathogens (e.g., Porphyromonas gingivalis (P.g), Tannerela forsythia (T.f), and Treponema dentico la (T.d)) as an example by separating their polymerase chain reaction products. Experiments demonstrated that P.g, T.d and T.f were diagnosed within 12 min, and the electrophoresis of P.g showed that the detection limit of this system was about 6.4 ng/mu l. Such a low cost system is easy to operate, and can effectively improve SGE in the biological experiment, especially for the labs in the third world countries. (C) 2017 Published by Elsevier B.V.