INACTIVATION OF PEROXIDASE BY HYDROGEN-PEROXIDE AND ITS PROTECTION BY A REDUCTANT AGENT
INACTIVATION OF PEROXIDASE BY HYDROGEN-PEROXIDE AND ITS PROTECTION BY A REDUCTANT AGENT
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DOI：
10.1016/0167-4838(90)90014-7
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发表时间：
1990-03-29
期刊：
影响因子：
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通讯作者：
GARCIACANOVAS, F
中科院分区：
文献类型：
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作者：
ARNAO, MB;ACOSTA, M;GARCIACANOVAS, F
过氧化氢是过氧化物酶的氧化剂底物，也是该酶的灭活剂。还原剂底物保护酶免于失活过程。提出了过氧化物酶化合物I存在两条竞争路径的反应机理；一种催化，一种失活。反应结束时产生的分析溶液支持所提出的机制，并显示了酶的转换次数 (r) 与两种底物的比率之间的依赖性。
Hydrogen peroxide, the oxidant substrate of peroxidase, is also an inactivating agent of this enzyme. The reductant substrates protect the enzyme from the inactivating process. A reaction mechanism is proposed, in which two competitive routes exist for Compound I of peroxidase; one catalytic and one inactivating. The analytical solution produced at the end of the reaction supports the proposed mechanism and shows the dependence between the number of turnovers of the enzyme (r) and the ratio of both substrates.