hPrimpol1/CCDC111 is a human DNA primase-polymerase required for the maintenance of genome integrity
hPrimpol1/CCDC111 is a human DNA primase-polymerase required for the maintenance of genome integrity
复制标题
hPrimpol1/CCDC111 是维持基因组完整性所需的人类 DNA 引物酶聚合酶。
DOI：
10.1038/embor.2013.159
复制
发表时间：
2013-12-01
期刊：
影响因子：
7.7
通讯作者：
Huang, Jun
中科院分区：
文献类型：
--
作者：
Wan, Li;Lou, Jiangman;Huang, Jun
Prim-pol是最近发现的一种DNA引物聚合酶，属于古细菌-真核生物引物酶（AEP）超家族。在这里，我们在人类细胞中描述了一种以前未被识别的引物酶，我们将其命名为hPrimpol1（人类引物聚合酶1）。hPrimpol1在体外具有引物酶和DNA聚合酶的活性，直接与RPA1相互作用，并以依赖RPA1的方式被招募到DNA损伤位点和停滞复制叉。缺乏hPrimpol1的细胞在重启停滞的复制叉时显示出更多的自发DNA损伤和缺陷。在DNA复制过程中，RPA1的结合和hPrimpol1的引物酶活性都是其细胞功能所必需的。我们的研究结果表明，hPrimpol1是参与DNA复制应激反应的新因子。
Prim-pol is a recently identified DNA primase-polymerase belonging to the archaeao-eukaryotic primase (AEP) superfamily. Here, we characterize a previously unrecognized prim-pol in human cells, which we designate hPrimpol1 (human primase-polymerase 1). hPrimpol1 possesses primase and DNA polymerase activities in vitro, interacts directly with RPA1 and is recruited to sites of DNA damage and stalled replication forks in an RPA1-dependent manner. Cells depleted of hPrimpol1 display increased spontaneous DNA damage and defects in the restart of stalled replication forks. Both RPA1 binding and the primase activity of hPrimpol1 are required for its cellular function during DNA replication. Our results indicate that hPrimpol1 is a novel factor involved in the response to DNA replication stress.